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MT6320 > 03: Haemophilus & Other Fastidious Bacteria 2 > Flashcards

03: Haemophilus & Other Fastidious Bacteria 2 Flashcards

1
Q

Identified in 1970s, caused pneumonia among elderly males due to contaminated air conditioning unit

A

Legionella

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2
Q

Catalase and oxidase reaction of Legionella

A

positive, positive

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3
Q

Other pathogens in close association with Legionella

A
  • Naegleria
  • Acanthamoeba
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4
Q

Legionella

Most spp. produce fluorescence after exposure to ___

A

UV light (366 nm)

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5
Q

Most clinically significant Legionella spp.

A

L. pneumophila

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6
Q

L. pneumophila

More severe clinical manifestation; causes community-acquired pneumonia

A

Legionnaire’s disease

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7
Q

L. pneumophila

Less severe clinical manifestation; non-pneumonic

A

Pontiac fever

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8
Q

Legionella spp.

Causes Pittsburgh pneumonia; partially acid-fast

A

L. micdadei

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9
Q

Legionella spp.

Causes Wiga agent pneumonia

fatal among renal failure px.

A

L. bozemanii

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10
Q

Legionella spp.

Causes pneumonia

A

L. dumoffi

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11
Q

Legionella spp.; lab. dx.

Sputum is treated with ___ to enhance recovery, decontaminate sputum, and inhibit normal flora

A

KCl + HCl

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12
Q

Legionella spp.; lab. dx.

Staining method for L. micdadei

A

Modified Kinyoun method

partially acid-fast

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13
Q

Legionella spp.; lab. dx.

Staining methods used

A

Silver staining; Giemsa

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14
Q

Legionella spp.; lab. dx.

Appearance of Legionella on BCYE with Cysteine

A

ground glass appearance

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15
Q

Legionella spp.; lab. dx.

Fluoresce yellow-green on DFA

A
  • L. pneumophila
  • L. micdadei
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16
Q

Legionella spp.; lab. dx.

Fluoresce blue-white on DFA

A
  • L. bozemanii
  • L. dumophi
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17
Q

DOC for Legionella

A

erythromycin

18
Q

Fastidious organisms that are gram (-), causes subacute bacterial endocarditis, and are transient flora of URT & oral cavity

A

HACEK group

  • Hemophilus
  • Aggregatibacter
  • Cardiobacterium hominis
  • Eikenella corrodens
  • Kingella
19
Q

HACEK Group

Oxidase, catalase, indole reaction

A
  • oxidase (+)
  • catalase (-)
  • indole (-)
20
Q

HACEK Group

Lactose fermenter

A

Aggregatibacter aprophilus

H. aprophilus

21
Q

HACEK Group

Catalase (+)

A

Aggregatibacter actinomycetemcomitans

all other spp. are catalase (-)

22
Q

HACEK Group

Indole (+)

A

Cardiobacterium hominis

all other spp. are indole (-)

23
Q

HACEK Group

Non-CHO fermenter

A

Eikenella corrodens

all other spp. are glucose fermenters

24
Q

HACEK Group

Nitrate (-)

A

Kingella kingae

25
Q

HACEK Group

Oxidase (-)

A

Capnocytophaga spp.

26
Q

HACEK Group

Oxidase positive

A
  • C. hominis
  • E. corrodens
  • K. kingae
27
Q

HACEK group

Loves high levels of CO2

A

A. aprophilus

28
Q

HACEK group

Causes endocarditis, head & neck infections

A

A. aprophilus

29
Q

HACEK group

Distinct odor of A. aprophilus

A

grade school paste

30
Q

HACEK group

Does not require X and V

A

A. aprophilus

31
Q

HACEK group

Virulence factor of A. actinomycetemcomitans

A

collagenase & leukotoxin

32
Q

HACEK group

Presence of a star shape (in LPO) with 4-6 points in the center of colonies after 48 hrs

A

A. actinomycetemcomitans

33
Q

HACEK group

Gram stain appearance of C. hominis

A

rosette formation; teadrop (dumbbell/ lollipop) appearance

34
Q

HACEK group

Causes periodontial disease

A

E. corrodens

35
Q

HACEK group

X factor; capnophilic

A

E. corrodens

36
Q

HACEK group

From human bite wounds, causes meningitis and endocarditis

A

E. corrodens

37
Q

HACEK group

Short, plump rods with squared ends

38
Q

HACEK group

Hemolytic pattern of K. kingae on BAP

A

B-hemolytic

39
Q

HACEK group

Important pathogen among pediatric population; predilection with bones and joints

40
Q

HACEK group

Often mistaken as N. gonorrhoeae as it is able to grow on modified Thayer Martin and causes endocarditis

A

K. denitrificans

MT6320 (10 decks)

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