Yeast as an expression host Flashcards
1
Q
What are the 4 types of yeast expression systems?
A
YIp
YEp
YRp
YCp
2
Q
Describe YIp
A
Yeast integrating plasmids that integrate into the host genome
3
Q
Describe YEp
A
Yeast episomal plasmids that are derived from endogenous yeast plasmids
4
Q
Describe YRp
A
Yeast replicating plasmids that contain endogenous autonomous replicating sequences derived from the yeast chromosome
5
Q
Describe YCp
A
Yeast centromere plasmids which contain a centromere from a chromosome and replicate like endogenous plasmids
6
Q
What is good and bad about YRp and YRp?
A
High copy number but unstable
7
Q
What is good and bad about YIp and YCp?
A
Low copy number but stable
8
Q
Describe Pichia pastoris
A
Methyltrophic yeast High expression Easy scale-up Inexpensive growth Does protein folding and processing Does post trans modifications Has integrating plasmids that are designed to integrate the expression construct containing the desired gene and selectable marker into the host via recombination Transormant is stable Does N-glycosylation
