Library construction Flashcards
1
Q
What is a genomic library?
A
A collection of clones that include all of the DNA sequences of a given species that can be screened for any clones containing the sequence of interest
2
Q
How are cDNA libraries made?
A
From isolated mRNAs
3
Q
How is cDNA made?
A
Reverse transcriptase makes DNA from RNA
Linkers are added to the ends of the DNA to help it join to the vector
