Degenerate PCR

Question 1

What are degenerate primers?

Answer 1

A pool of primers that bind to all variations of the target sequence

Question 2

How are the primers designed?

Answer 2

Use amino acids that are coded for by 2 codons (C, D, E, F, H, K, N, Y) or 1 codon (M, W) to estimate what the triplet codon sequence is

Question 3

What is codon bias?

Answer 3

Organisms will use certain codons over others to make amino acids