Functional genomics Flashcards
1
Q
What is functional genomics?
A
Understanding biological functions through the use of genomic/transcriptomic data
Investigation of cell functions
Assigns functions to genes
2
Q
What are some methods in transcriptomics?
A
Northern blots in situ hybridisation RT-qPCR digital droplet PCR Microarray RNA-seq
3
Q
What are some methods in proteomics?
A
2DE-MS LC/MS Metalloproteomics Phosphoproteomics Structural genomics
4
Q
What is the transcriptome?
A
The set of all the mRNA in a cell
5
Q
What are the discrepancies between protein and mRNA in cells?
A
mRNA levels do not always correlate with protein levels in a cell
6
Q
What are protein arrays used for?
A
Detection of biomarkers
Protein quantification in cell lysate
Protein: protein interactions
Antibody characterisation
