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BIO2030 Biotechnology > Detecting recombinant proteins > Flashcards

Detecting recombinant proteins Flashcards

1
Q

Describe electrophoresis

A

Separation based on charge
8.3-8.6 pH
DNA moves towards the anode
Needs support medium ie starch/agarose

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2
Q

Describe PAGE

A

Uses polyacrylamide gel
e- flow is even
Mobility based on charge and gel pore size

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3
Q

Describe SDS PAGE

A

Sodium dodecyl sulfate unfolds the protein and coats it with a negative charge
Charge:mass the same for all proteins
Mobility based on mass
Sieving so small proteins are at the front

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4
Q

What are some ways to purify proteins?

A

Column chromatography
Size exclusion chromatography
Ion exchange chromatography
Affinity chromatography

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5
Q

What is affinity chromatography based on?

A

Antibody:antigen
Enzyme:inhibitor

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BIO2030 Biotechnology (34 decks)

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