Week 9 Tuesday

Question 1

What are the two main parts of co-transformation?

Answer 1

Transform a CRISPR plasmid that will cleave the genome and an editing template that will stimulate the editing that we want

Question 2

The promoter transcribes the ____.

Answer 2

spacer

Question 3

What is the purpose of delivering a CRISPR RNA?

Answer 3

is to transcribe a piece of the DNA into RNA. That RNA is binding some target on the yeast genome and that will stimulate CAS9 which is the scissors that cut the genome.

Question 4

Why did we use three different target regions on the S. cerevisiae genome?

Answer 4

In case there happens to be a mutation in our yeast strain where one of them doesn’t work.Or maybe one will work better.

Question 5

How did we create a linear repair template?

Answer 5

PCR

Question 6

Why did we split the linear repair template?

Answer 6

Because 1 is too big. PCR revolves around a polymerase that extends the DNA, after a while of adding nucleotides, it gets tired and starts to make mistakes.

Question 7

True/False: Shorter PCRs are more accurate.

Answer 7

True

Question 8

What is the purpose of snaps?

Answer 8

recombine the template into one piece after transformation

Question 9

Where does the TALE on the primer bind?

Answer 9

The yeast genome
The TALE helps to find where to bind to the genome

Question 10

How do you test that the co-transformation achieved genome editing?

Answer 10

plating and seeing if it is orange