Week 11 Tuesday Flashcards
1
Q
What should we measure to get a full picture of our strain’s performance?
A
Product (metabolite of interest), Intermediate metabolite, Side product, On-pathway flux, Off-pathway flux
2
Q
Give EX of Product (metabolite of interest).
A
beta-carotene
3
Q
Give EX of Intermediate metabolite.
A
GGPP, phytoene, neurosporene, lycopene, etc
4
Q
Give EX of Side product.
A
7,8-dihydro-beta-carotene, etc
5
Q
Give EX of On-pathway flux.
A
Mevalonate, IPP, DMAPP, GPP, FPP, etc…
6
Q
Give EX of Off-pathway flux.
A
Squalene, monoterpenes, ergosterol, etc…
7
Q
How can we detect and measure multiple metabolites within the same sample?
A
LCMS
8
Q
How does LCMS data work?
A
correlates intensity with concentration (MS)
9
Q
A
