Trivia Flashcards
1
Q
CRISPR uses what type of molecule to bind the “spacer” region of target DNA?
A
crRNA
2
Q
What is the function of Cas9 in CRISPR-based genome editing?
A
Binding the PAM DNA sequence and Cleaving DNA
3
Q
What did we add into our plasmid using Golden-Gate cloning?
A
DNA to encode a crRNA
4
Q
How is our beta carotene genome editing template encoded?
A
On a linear template split into two parts
5
Q
How many genes are encoded on our beta carotene pathway template?
A
4
6
Q
What is a benefit of genome editing compared to expressing genes on a plasmid?
A
More stable and robust growing strain
7
Q
Why would we use cells to produce chemicals?
A
Less carbon emissions and Less land utilized
8
Q
Which fields can Synthetic Biology be applied to?
A
Agriculture
Energy
Pharmaceutical
Chemical
9
Q
A
