Week 3 wednesday

Question 1

What is the native software?

Answer 1

Yeast metabolism

Question 2

What is the novel software?

Answer 2

Plasmid-encoded enzymes

Question 3

What did we use to neutralize the environment for S. cerevisiae?

Answer 3

lithium acetate

Question 4

What does antibiotic markers entail?

Answer 4

select by growing cells in media with added antibiotic, where the plasmid makes cells resistant

Question 5

What do auxotrophic selection markers entail?

Answer 5

select by growing cells in media deficient in a single nutrient, where the plasmid contains the key to making the nutrient