Week 8 Tuesday Flashcards
1
Q
What are the limitations of generating a beta carotene-producing yeast strain by transforming a plasmid expressing the beta carotene pathway
A
expensive selection media, strain robustness (poorer growth) & stability (potential to cure plasmid)
2
Q
What are the limitations of Integrating the beta carotene pathway into the genome of S. cerevisiae?
A
Required strain engineering is more difficult, potential to disrupt important part of genome, lower expression?
3
Q
True/False: double-stranded breaks in the genome are lethal unless repaired by the cell
A
True
4
Q
A
