Week 2 Random Flashcards
Timing of migration of Primordial germ cells (PGCs)
2nd week: formed in epiblast
3rd week: move to the wall of the yolk sac
4th week: migrate to the developing gonads
5th week: arrive in the gonads then meiosis In the mouse, ~100 PGCs leave the yolk sac →6-7 rounds of mitotic multiplication →4000 enter the primitive gonads
Fate of misdirected PGCs
Die or Form Teratomas
Stages of formation of gametes
1) Movement of PGC to gonads
2) Mitosis in gonads (overproduction followed by density mechanism) in oogonia divide only during development, and in males spermatogonia divide during development and puberty
3) Meiosis
4) Structural and Functional maturation of Eggs and Sperm Oogenesis and Spermatogenesis
Oogenesis
2 million primary oocytes present in the ovaries at birth, only ~40,000-survivie until puberty.
400 (1 per menstrual cycle) are ovulated. Primary oocytes remain in prophase and do not finish their first meiotic division before puberty is reached.
At puberty, one activated primary oocyte produces two haploid cells asymmetric cell division The first polar body The secondary oocyte
The secondary oocyte arrests in metaphase II and is ovulated If penetrated by sperm the second oocyte completes meiosis II, One large ovum (the functional gamete) A tiny second polar body
Describe spermatogenesis
Spermatogonia (diploid)
Spermatocytes (enter meiosis)
Spermatids (haploid)
->spermiogenesis-> during which they acquire a tail (flagellum) and an acrosome that develops from the Golgi body
Sperm
Spermiogenesis:
- formation of acrosome (penetration enzymes)
- condensation of nucleus
- formation of neck, middle piece, and tail
- shedding of most of the cytoplasm that is phagocytized by Sertoli cells
Spermiation
Excess cytoplasm and is released into the lumen of the seminiferous tubule
How Kareotype is done
Leukocyte are isolated, propagated, and toxin are injected Cells are squashed on microscope slide Lightly treated with protease to unwind DNA Stained with Geimsa (non-specific) stain- (total 850 bands; about 50/csome)
Position of centromere
Metacentric (middle) submetacentric- towards one end acrocentric - at the end
p vs q
p is short arm q is long arm 7p22.2 46,XY,inv(9).(p11q12)
FISH
fluorescence in situ hybridization Specific nucleotide probes abeled with fluorescent dyes can be hybridized to the solated chromosomes or nuclei on microscope slides and nalyzed by fluorescence microscopy.
Types of probes in FISH
Locus specific (label single gene or region) Centromeric probe (labels a specific pair of chromosomes at their centromere) Chromosome painting probe (paint entire chromosome)
Two preps for FISH
Metaphase nuclei: visualize chromosome structure Interphase nuclei: determine number
SKY
Spectral Karyotiping FISH with different colors Use in cancer treatment
Why chromosomal studies are important
Confirmation/determination of diagnosis Prognosis Treatment Monitoring disease – disease progresion and relapse
Microarray SNP
This analysis is designed to look for imbalances across the genome using SNP (Single Nucleotide Polymorphism) based technology. The SNP microarray testing has probes that can detect imbalances related to hundreds of common Balanced or low level mosaic chromosome changes will not be identified with this technology
Use of Cell Free Fetal DNA
- Maternal age 35 years or older at delivery - Fetal ultrasonographic findings indicating an increased risk of aneuploidy - History of a prior pregnancy with a trisomy - Positive test result for aneuploidy, including first trimester, sequential, or integrated screen, or a quadruple screen. - Parental balanced robertsonian translocation with increased risk of fetal trisomy 13 or trisomy 21. - 10-15% DNA in mother blood is free fetal DNA - The goal of the test is very specifc trisome 13, 18, 21, XO, XXY
false positive false negative
Type 1 error, 1-accuracy Type 2 error, 1-sensitivty
Balanced / Unbalanced abnormalities of chromosomes
Duplications Inversions Inertions Isochromosomes
der(14;21)
Chromosome derived from 14 and 21
Balanced Translocations types
Reciprocal translocation Parts of chromosomes are exchanged Can result in mutations at break points or can be unaffected. Problems with segregation at gametogenesis Robertsonian translocation- centric fusion of two chromosomes. No lost coding sequences, carrier unaffected. Problems with segregation at gametogenesis.
Balanced vs. Unbalanced chromosomal rearrangements chraracteristic and types
Usually lethal 1) Spontaneous new mutation 2) Unbalanced inheritance from balanced rearrangements
Reciprocal translocation
Balanced chromosomes exchange
chromosomal studies can help with
Diagnosis (confirmation/determination) Prognosis Recurrence Risk
Amniocentesis
a prenatal diagnosis of chromosomal abnormalities and fetal infections, and also used for sex determination in which a small amount of amniotic fluid, which contains fetal tissues, is sampled from the amniotic sac surrounding a developing fetus, and the fetal DNA is examined for genetic abnormalities
Cordocentesis
highly specialized prenatal test in which a sample of the baby’s blood is removed from the umbilical cord for testing
Cytogenetics techniques
1) Karyotyping
2) FISH
3) Microarray SNP Analysis
4) Cell free fetal DNA in Maternal Plasma
How does FSH effect ovary?
Production of estrogen
What are the functions of Estrogen?
Proliferation of endometrium
Thinning of the cervical mucus
Stimulate pituitary to secrete LH
LH surge at mid-cycle-> ovulation
Stimulate progesterone production
What cells that produce estrogen in female in ovary?
granulosa and thecal cells
What are LH surge effects?
completion of meiosis I -> preovulatory follicle; arrested in metaphase of meiosis II: 3hrs before ovulation
On ovarian surface:
LH↑→Collagenase↑→Digestions of fibers around the follicle
LH↑→Prostaglandin↑→muscular contraction
What are cells and their function in Corpus lueteum?
Lutean cells: granulosa cells, thecal cells
Progesterone + Estrogen (turns uterine into secretion phase)
How long does it take for an egg to go from ampulla to uterine lumen?
3-4 days
Where does the fertilization occur?
At the ampula of uterine tube
What secretes hCG?
syncytiotrophoblast
When does the corpus luteum stop to produce progesterone?
nProgesterone production until the end of 4th month: trophoblastic secretion of progesterone becomes adequate.
Name three phases of fertilization
Phase I: penetration of the corona radiata
Phase II: penetration of zona pellucida
Phase III: Fusion of the oocyte ad sperm cell membranes
What is Capacitation?
(wash the sperm by folliciular tubule): Maturation of sperm in female reproductive tractà Removal of the glycoprotein coat and seminal plasma proteins from the sperm plasma membrane (Important for IVF invitro fertiliation and IUI)
What is Acrosome reaction?
Release of enzyme by sperm to dissolve zona pellucida (ZP)
What events are triggered by entry of sperm to an egg?
Cortical and zona reactions: to prevent polyspermy (forming cortical granules)
Oocyte completes meiosis II
Egg is activated
What are Blastomeres?
2-, 4-, 8-cell stages, cells →smaller and smaller
What are the results of fertilization?
Restoration of diploid number of chromosomes
Sex determination (at fertilization)
Initiation of cleavage ge:en-US’>Cell free fetal DNA in Maternal Plasma
How does Blastocyst integrate in uterus?
ZP degenerates → uterine fluid penetration → Blastocele (cavity) formation
Inner cell mass → embryoblast
outer cell mass → trophoblast, epithelial wall
What are the three epithelial layers in endometrium?
Compact layer, spongy layer, and basal layer
Contraceptive methods:
Barrier
Hormone-based
IUD nRU-486 (mifepristone): potent progesterone antagonist nSurgery: Vasectomy & tubal ligation
trophoblast differentiation
Week 2 Day 8
Cytotrophoblast (mononucleated) migrate in the Syncitotrophoblast region and lose connection
Syncytiotrophoblast (multinucleated without border)
When and how embroyblast differentiate?
Week 2 Day 8
Hypoblast layer (adjacent to blastocyst cavity)
Epiblast layer (adjacent to the amniotic cavity)
When and where does the amniotic cavity form?
Week 2 Day 8
Surrounded by epiblast cells
When and what makes amniotic memberane?
Week 2 Day 8
Amnioblast (epiblast cells that adjacent to the cytotrophoblasts)
What are lacunae? How do they form? Where do they appear? When do they appear?
Week 2 Day 9
Vacuoles fuse in syncytiotrophoblast
What types of cells are present in hypoblast and epiblast during Day 9?
Epiblast – columnar
Hypoblast – cuboidal
What is fibrin coagulum?
A plug that seal the outer layer of endometrium after the embryo implants in uretus.
When does the lacuna becomes continous with sinusoids?
Week 2 Day 11-12
What is the name of a layer that surrounds Secondary yolk sac?
What is the name of a layer that surrounds amniotic cavity and chorionic cavity?
When do they appear?
Week 2 Day 11-12
Extraembryonic splanchnic mesoderm.
Extraembryonic somatic mesoderm.
What is the name of the cavity that is surrounded by extrasomatric membrane?
Chorionic caivty
What are the extension of cytotrophoblast called?
When and where do they grow?
Week 2 Day 13
Primary villi
They grow in syncytiotrophoblast
When and where does the secondary yolk sac come from?
Week 2
It pinches off from the primitive sac.
Also called definitive yolk sac.
What is the exocoelomic cavity?