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Animal Physiology > Water and Ion balance > Flashcards

Water and Ion balance Flashcards

1
Q

What is the extracellular fluid made of?

A

80% interstitial fluid and 20% plasma

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2
Q

What is the main extracellular cation?

A

Sodium

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3
Q

What is the main intracellular cation?

A

Potassium

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4
Q

What is the main extracellular anion?

A

Chloride

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5
Q

What are the 5 ions that are super important for ion balance?

A

Sodium, potassium, chloride, magnesium, calcium

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6
Q

What is osmolarity?

A

The number of moles of a solute that contributes to the osmotic pressure of a solution

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7
Q

What is the osmolarity of sea water?

A

1000 mOsM

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8
Q

What is the osmolarity of freshwater?

A

50 mOsM

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9
Q

What is tonicity?

A

Properties of an extracellular solution of impenetrable solutes that determine if water will move into or out of a system (a cell), measures the osmotic pressure gradient between 2 solutions

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10
Q

Why do cells act as osmometers?

A

They swell or shrink in response to osmotic gradients but can regulate their transport to maintain a constant volume

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11
Q

What happens to a cell in a more dilute solution? How does the cell get its volume back to normal?

A

(hypotonic solution) Water enters the cell through osmosis and the cell swells up. A cell will export solutes to get some of the water to leave

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12
Q

What happens to a cell in a more concentrated solution? How does the cell get its volume back to normal?

A

(hypertonic solution) Water leaves the cell through osmosis and the cell shrinks. A cell will import solutes to get some water to come in

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13
Q

Do only inorganic ions contribute to osmotic gradients?

A

No, cells will also alter their organic solute concentration to regulate their volume

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14
Q

What happens to the amount of organic solutes in the intracellular fluid if osmotic pressure increases?

A

Increases

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15
Q

What does the Ussing chamber do?

A

Tests ion transport across a semipermeable membrane by manipulating the chemistry on one side and measuring transport

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16
Q

What does an Ussing chamber experiment tell us about the transporters on the membrane?

A

Which side of the cells the transporters are on and if the transport is active or passive

17
Q

What were the 5 results obtained from Ussing and Huf’s experiment with the Ussing chamber and the frog skin?

A
  1. Sodium transportation happens against the concentration gradient
  2. Na+ transport is blocked if ATP metabolism is blocked
  3. Transport is temperature dependent
  4. Transport shows saturation kinetics
  5. Transport is solute specific
18
Q

What 2 chemicals inhibit ATP metabolism?

A

Ouabain (blocks ATPase function), and cyanide (blocks oxidative phosphorylation)

19
Q

What were the 3 conclusions drawn by Ussing and Huf after their experiment with frog skin in an Ussing chamber?

A
  1. Ouabain inhibited transport on the basolateral side, but not the apical side, so the ATPase was only present on the basolateral side
  2. K+ ions are present on the basolateral side but not on the apical side, not necessary there
  3. Na+ saturation kinetics only exist on the apical side, only carriers there instead of ATPases
20
Q

Which membrane are Na+ carriers and channels located on?

A

Apical

21
Q

Which membrane is the Na+/K+ ATPase located on?

A

Basolateral

22
Q

What is the difference between obligatory and controlled osmotic changes?

A

Obligatory changes come from the environment and we can’t control them, controlled osmotic changes are caused by active transport and are homeostatic mechanisms

23
Q

What are 4 things that cause obligatory osmotic changes?

A
  1. Metabolic water production
  2. Diffusion across epithelium
  3. Defecation
  4. Ingestion
24
Q

What does the water transport need to be to maintain homeostasis?

A

Input needs to equal output

25
Q

What is the internal pool of a substrate?

A

The amount of that substrate in the extracellular fluid

26
Q

What happens to maintain homeostasis when there is an addition to the internal pool of a substance?

A

There needs to be an output as well

27
Q

What 3 things will are input sources of water?

A

Food, drinks, metabolism

28
Q

What 4 things are output sources of water?

A

Feces, kidneys, skin, lungs

29
Q

What are the 6 factors that affect obligatory osmotic exchange?

A
  1. Gradient between extracellular compartment and the environment
  2. Surface area to volume ratio
  3. Permeability of the membrane
  4. Feeding (salt and water intake)
  5. Temperature, exercise, respiration
  6. Metabolic factors
30
Q

How much oxygen is required to break down food? How much water gets produced?

A

Depends on the type of macromolecule. Catabolism of carbs and proteins produces less water and uses less oxygen, but also produces less energy than fat breakdown

31
Q

What is produced by the catabolism of food?

A

Monomers of whatever got broken down (amino acids, fatty acids, or monosaccharides) and water

Animal Physiology (32 decks)

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