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special stains > von kossa > Flashcards

von kossa Flashcards

1
Q

purpose/target of von kossa

A

demonstrate calcium

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2
Q

mechanism/principle of von kossa

A

indirect staining. silver does not bind to calcium directly, it binds to the phosphate and carbonate of the salts

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3
Q

control tissue for von kossa

A

bone that has not been decalcified, & anything with calcium deposits

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4
Q

counter stain for von kossa

A

nuclear fast red

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5
Q

nuclear fast red is the ____ stain for von kossa?

A

counter stain

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6
Q

what colour is the background in von kossa

A

red/pink

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7
Q

what color is the target in von kossa

A

black/brown

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8
Q

how is silver reduced in von kossa?

A

bright/uv light

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9
Q

purpose of bright or uv light in von kossa?

A

reduce silver into metallic silver

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10
Q

purpose of sodium thiosulfate in von kossa

A

remove unreduced silver

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11
Q

how to remove unreduced silver in von kossa?

A

sodium thiosulfate

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12
Q

working solution in von kossa?

A

silver nitrate. binds with calcium salts

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special stains (19 decks)

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