gordon n sweets

Question 1

target/purpose of gordon & sweets stain

Answer 1

reticulin fibers

Question 2

method/principle of staining for gordon & sweets stain

Answer 2

silver impregnation / argyrophil method

Question 3

control tissues for gordon & sweets

Answer 3

liver, kidney

Question 4

what color will the target stain in gordon & sweets?

Answer 4

reticulin=black

Question 5

counterstain in gordon & sweets?

Answer 5

nuclear fast red

Question 6

what colour will the background be in gordon and sweets?

Answer 6

pink/red

Question 7

steps in the gordon and sweets stain?

Answer 7

-oxidize tissue with potassium permanganate
-bleach tissue in oxalic acid (turns it colourless)
-sensitize tissue with iron alum/ferric ammonium sulfate
-impregnate in silver solution
-reduce silver using 10% formalin
-tone silver w gold chloride
-remove unreduced silver sodium thiosulphate
-counterstain w/ nuclear fast red

Question 8

purpose of potassium permanganate in gordon and sweets stain?

Answer 8

oxidize tissue

Question 9

what substance oxidizes tissue in gordon and sweets stain?

Answer 9

potassium permanganate

Question 10

purpose of oxalic acid in gordon and sweets stain?

Answer 10

bleach tissue

Question 11

what substance bleaches tissue in gordon and sweets stain?

Answer 11

oxalic acid

Question 12

purpose of ferric ammonium sulfate in gordon and sweets stain?

Answer 12

sensitizes tissue

Question 13

what substance sensitizes the tissue in gordon and sweets stain?

Answer 13

ferric ammonium sulfate

Question 14

does gordon and sweets use the argyrophilic or argentaffin method of silver impregnation?

Answer 14

argyrophilic

Question 15

what is the reduction agent for gordon and sweets?

Answer 15

10% formalin