masson fontana

Question 1

purpose/target of masson fontana?

Answer 1

demonstrate argentaffin substances (e.g. melanin, neurosecretory granules)

Question 2

counterstain of masson fontana

Answer 2

nuclear fast red

Question 3

nuclear fast red is the _____ stain in masson fontana?

Answer 3

counterstain

Question 4

principle/mechanism of staining for masson fontana

Answer 4

argentaffin metallic impregnation

Question 5

how is the silver reduced in masson fontana?

Answer 5

does not need a reducing agent because argentaffin substances will reduce it by themselves

Question 6

control tissue for masson fontana

Answer 6

skin, small intestine, appendix

Question 7

what colour will melanin and argentaffin granules stain in masson fontana

Answer 7

black

Question 8

what colour is the background in masson fontana

Answer 8

red/pink

Question 9

masson fontana is good for staining what kind of tumours

Answer 9

carcinoid tumours because they have neurosecretory granules and argentaffin granules

Question 10

purpose of gold chloride in masson fontana

Answer 10

toning

Question 11

purpose of sodium thiosulphate in masson fontana

Answer 11

remove unreduced silver

Question 12

what is the working solution in masson fontana?

Answer 12

Fontana Solution (ammoniacal silver)

Question 13

purpose of gold chloride in Masson fontana

Answer 13

tones silver