periodic acid schiff Flashcards
1
Q
purpose/target of PAS
A
neutral carbs/polysaccharides, neutral mucosubstances, glycogen
2
Q
counterstain for PAS
A
hematoxylin or fast green for fungus
3
Q
which fixative to avoid for PAS?
A
avoid glutaraldehyde
4
Q
good controls and bad control tissue for PAS?
A
good = kidney, cervix. should have a “with” and “without” diastase control
bad= liver. has too much glycogen
5
Q
what colour does the target stain PAS
A
glycogen=magenta
6
Q
oxidizing agent in PAS?
A
periodic acid. polysaccharides are now aldehydes and binds to schiff reagent
7
Q
results on the diastase slide of PASd?
A
no magenta, because the diastase enzyme has digested/broken down all the glycogen
