congo red

Question 1

purpose/target for congo red

Answer 1

amyloid

Question 2

counterstain for congo red

Answer 2

hematoxylin

Question 3

hematoxylin is the ___ stain for congo red

Answer 3

counter stain

Question 4

principle/method of dyeing in congo red?

Answer 4

hydrogen bonding to the cellulose in amyloid

Question 5

control tissue for congo red

Answer 5

anything with amyloid

Question 6

what colour does the target stain in congo red

Answer 6

salmon pink in light microscope / apple green birefringent polarizing microscopy

Question 7

section thickness for congo red

Answer 7

8-10 um

Question 8

if section is too thin in congo red?

Answer 8

amyloid looks red

Question 9

section too thick in congo red?

Answer 9

yellow birefringence

Question 10

what substance to add to enhance the hydrogen bonding between dye and amyloid?

Answer 10

sodium chloride (NaCl salt!)

Question 11

primary stain in congo red?

Answer 11

congo red solution

Question 12

what is alkaline salt solution for in congo red?

Answer 12

release internal hydrogen bonds on protein chains

Question 13

what solution will release internal hydrogen bonds on protein chains in congo red?

Answer 13

alkaline salt solution

Question 14

what colour will elastic fibers be in congo red

Answer 14

pale pink