masson trichrome

Question 1

purpose/target of masson trichrome

Answer 1

demonstrate collagen

Question 2

counterstain of masson trichrome

Answer 2

aniline blue OR light green
(light green used when collagen is dominant)

Question 3

purpose of Bouin’s solution in masson trichrome

Answer 3

preferred fixative instead of 10% formalin.
if tissue fixed in formalin, Bouin is used as MORDANT

Question 4

what colour do these stain in masson trichrome?
-nuclei?
-cytoplasm, keratin, muscle?
-collagen, mucin?

Answer 4

-nuclei =black
-cytoplasm, keratin, muscle = red
-collagen, mucin = blue (aniline blue)

Question 5

principle/mechanism of staining for masson trichrome

Answer 5

porosity and size of dye molecules

Question 6

phosphotungstic acid purpose in masson trichrome?

Answer 6

differentiates

Question 7

what is Bouin’s solution in masson trichrome made of?

Answer 7

Bouin solution is mix of picric acid, formalin, acetic acid

Question 8

what 3 stains is masson trichrome stain made of?

Answer 8

-weigert’s hematoxylin
-acid dye Biebrich scarlet acid fuchsin
-aniline blue

Question 9

what stain uses Weigert’s hematoxylin?

Answer 9

masson trichrome

Question 10

what stain uses Biebrich scarlet acid-fuchsin and aniline blue?

Answer 10

masson trichrome