perl's prussian blue Flashcards
1
Q
purpose/target of Perl’s
A
demonstrate ferric iron
2
Q
counterstain of Perl’s
A
neutral red / safranin
3
Q
neutral red or safranin is the _______ stain in Perl’s?
A
counterstain
4
Q
background colour of slide in Perl’s?
A
red/pink
5
Q
what colour does the target stain in Perl’s
A
blue
6
Q
control tissue for Perl’s
A
spleen, liver, bone marrow, RBC. NOT THE HEART
7
Q
Primary stain/working solution in Perl’s
A
potassium ferrocyanide + HCl
8
Q
what does HCl do in perl’s
A
acidifying solution
9
Q
what is the final pigment called in Perl’s
A
ferri-ferrocyanide
10
Q
what is ferri-ferrocyanide in Perl’s
A
final pigment after ferric iron binds with potassium ferrocyanide
11
Q
why doesn’t hemoglobin react in Perl’s even though its iron?
A
too strongly bound to its protein
