Virology/Microbiology

Question 1

What is the enzyme called which makes mRNA?

Answer 1

DNA dependent RNA polymerase

can be host DNA or viral DNA

Question 2

What are the 7 classes of viral genome?

Answer 2

dsDNA
gapped dsDNA
ssDNA
dsRNA
ss (+) RNA
ss (-) RNA
ss (+) RNA with DNA intermediate

Question 3

What does the enzyme reverse transcriptase do?

Answer 3

Converts RNA into dsDNA. This dsDNA then forms part of the host DNA.

Question 4

Gram positive cocci include?

Answer 4

Staph
- S. aureus/S epidermidis/CONS
Strep
- GAS (pyogenes)/GBS/GDS (enterococcus fecalis)/Group E onwards

Question 5

Gram negative cocci?

Answer 5

Neisseria (Gonococcus/meningitidis)

Question 6

Gram positive rods?

Answer 6

Diphtheria
Listeria
Others (Norcardia, bacillus, clostridia, mycobacteria)

Question 7

Which bacteria appear blue on gram stain?

Answer 7

gram positive bacteria (cell wall able to take up gram stain)

Question 8

How do “lytic” viruses work?

Answer 8

kill host cell at release (polio, influenzae)

Question 9

How is bacterial replication described?

Answer 9

binary fission

Question 10

How do viruses replicate?

Answer 10

All viruses prodcue mRNA which is translated by host ribosomes. Host machinery then replicates virus (viruses cannot replicate without a host)