Virology Final Dx

Question 1

CSF/ hog cholera

Answer 1

Notifiable. Lab Confirm. IF/Ag ELISA, Mab to diff from BVD, isolation, SN

Question 2

ASF Tests

Answer 2

Notifiable, Lab Confirm. VI to dif. CSF. Ag ELISA, IF

Question 3

Pseudorabies

Answer 3

History/CS. Conf. W/ Ab ELISA, IF, VI, SN isolation.

Question 4

PMWS

Answer 4

History/CS/InBs. Confirm w/ VI, PCR

Question 5

Swine Influenza

Answer 5

Lab confirm – VI = gold IF. Mab typing, RT- PCR

Question 6

PRRS

Answer 6

Moribund Live Pigs for VI

Question 7

Porcine Parvo

Answer 7

In fetus, IH, IF, PCR. Serology has limited value. Dz difficult to detect in early preg.

Question 8

TGE

Answer 8

Intestinal tissue isolation w. SN, retrospective diagnosis by paired sera

Question 9

swine vesicular Dz

Answer 9

Ag ELISA, RT-PCR, VI

Question 10

EIA

Answer 10

Sero (Coggins -(FN careful) & ELISA), PCR for proviral DNA

Question 11

EVA

Answer 11

RT-PCR, Ag ELISA, VI cell culture w/ IF

Question 12

Eq. Encephalities

Answer 12

IgM capture ELISA w/ neut. To confirm. Postmortem RT-PCR of brain tissue

Question 13

Eq. EEE/WEE

Answer 13

Ab ELISA, Neut. Assay

Question 14

West Nile

Answer 14

RT-PCR, IHC and IF, VI, Sero (IgM ELISA, neut, compliment fixation)

Question 15

Equine influenza

Answer 15

VI, HA/HI, retro serological testing

Question 16

<p>Eq. Rhinopneumonitis</p>

Answer 16

PCR, IF of ag, Sero, VI

Question 17

Adenovirus Pneumonia

Answer 17

Ag ELISA, PCR, Sero (HI & neut), VI

Question 18

Eq. Abortion

Answer 18

InIBs, VI, PCR, IF, Paired serum

Question 19

Eq. Coital Exanthema

Answer 19

PCR, Sero, EM

Question 20

Vesicular Stomatitis

Answer 20

VI, sero

Question 21

Newcastle Dz

Answer 21

Lab confrim (notifiable), VI- spleen, brain, lungs. IF – tracheal secretions (less sensitive). Ab detection w/ HI– in unvaccinated flocks

Question 22

IBD

Answer 22

History/CS, necropsy, IF or IHC, RT-PCR, VI, sero (Ab ELISA neut.)

Question 23

Marek’s Dz

Answer 23

History/CS, characteristics of tumor cell, virological characteristics, Necropsy

Question 24

Avian Leukosis

Answer 24

History/CS, location of tumor, histopathology

Question 25

Avian Influenza

Answer 25

VI, Typing – HA/NA- inhibition. Humans – Ag (IF or ELISA)

Question 26

Avian infectious bronchitis

Answer 26

Direct IF of tracheal smears, VI, RT-PCR, types- sero

Question 27

Avian infectious laringotracheitis

Answer 27

CS/postmortum findings, IF, Sero (ELISA, Neut. )

Question 28

Chicken Anemia Virus

Answer 28

<p>Clinical, gross and histo, IF, EM, PCR, Neutralise, isolation to confirm</p>

Question 29

Avian reovirus

Answer 29

IF, Isolation followed by neut.

Question 30

Fowlpox

Answer 30

Histo, EM isolation. (cutaneous from clinical Dx only