Virology Exam 2 - Parvoviridae & Circoviridae

Question 1

the importance of rapidly dividing cells in pathogenesis of parvovirus

Answer 1

replication in nucleus but no DNA polymerase so it requires mitosis S phase

then replicates in intestinal crypt cells that lead to destruction and hemorrhage

Question 2

parvovirus diagnostic testing (esp. CPV-2)

Answer 2

SNAP ELISA (preferred) - ANTIGEN
PCR
virus isolation
serology (hemagglutination inhibition test) - sialic acid residues

Question 3

importance of non-enveloped viruses and difficulties for prevention/control

Answer 3

hardy in the environment
most disinfectants not effective, often require certain contact time or isolation

Question 4

Circoviridae
SHAPE
ENVELOPED/NON-ENVELOPED
DNA/RNA

Answer 4

icosahedral
non-enveloped
circular ssDNA

Question 5

Porcine Circovirus 1 & 3

Answer 5

unimportant

Question 6

Porcine Circovirus 2 (PCV2)
HOST
DISEASE

Answer 6

domestic swine, wild boar
associated with porcine circovirus associated disease (PCVAD)
porcine dermatitis nephropathy syndrome, reproducitve failure, postweaning multisystemic wasting syndrome, porcine respiratory disease complex

Question 7

Canine Circovirus (DogCV)
HOST
DISEASE

Answer 7

canines
vasculitis/vascular necrosis, hemorrhage in GI, liver, spleen, kidneys, lungs, granulomatous lymphadenitis of mesenteric LN

Question 8

Psittacine beak & feather disease virus
HOST
DISEASE

Answer 8

cockatoos, parrots, budgerigars
dystrophic feathers/beak, abnormal plumage or color, leukopenia/immunosuppression

Question 9

chicken anemia virus
HOST
DISEASE

Answer 9

chickens
immunosuppression, anemia, hemorrhage, anorexia, lethargy, depression, BLUE WING DISEASE

Question 10

canine parvovirus 2 (CPV2)
HOST
DISEASE

Answer 10

canines
myocarditis/cardiomyopathy, pulmonary edema, sudden death, panleukopenia, vomit, bloody diarrhea, hemorrhagic enteritis, lethargic, fever or hypothermia, secondary bacteremia

Question 11

feline panleukopenia virus (FPV)
HOST
DISEASE

Answer 11

felines
panleukopenia (low WBC, NEU, LYM), feline distemper, feline enteritis, feline ataxia “cat fever”, cerebellar hypoplasia

Question 12

porcine parvovirus (PPV)
HOST
DISEASE

what is this often co-infected with?

Answer 12

domestic swine
SMEDI

often co-infected with porcine circovirus 2 (PCV2)

Question 13

aleutian mink disease virus
HOST
DISEASE

Answer 13

mink, ferrets, skunks, raccoons
neonatal interstitial pneumonia, adults have persistent chronic immune-complex-mediated disease

Question 14

canine parvovirus 1 (CPV1)
HOST
DISEASE

Answer 14

canines
interstitial pneumonia, mild enteritis, fetal death, abortions

minute virus & genetically distinct from CPV2

Question 15

Parvovirus
SHAPE
ENVELOPED/NON-ENVELOPED
DNA/RNA

Answer 15

icosahedral
non-enveloped
linear ssDNA