Viral P, L and CH Flashcards
Role of P
defense and targeting of the genome
shape of the virion
enzymes for multiplication
receptors
Grouping of P
structural and non-structural
Structural proteins grouping
surface and core P
Surface and core P are what kind of P?
Structural
Where do we find surface proteins and what it their role?
capsid and envelope
shape, adsorption, HA activity, E for penetration or release of virions, antigenicity
Where do we find the core P and what it their role?
in the nuclei (core)
NA protection, stabilization, E for replication
Non-structural proteins grouping
early, immediate early P and late P
Non-structural P description
coded in the viral genome
not integrated in the virion
only in the vegetative virus
The functions of the early, immediate early proteins is?
regulation of the cell, inhibition of cell. defense, E for replication
Function of the late P?
E for structural P maturation, Virus assembly
The majority of the late P is structural
YES!
We can find early P in the virion too
FALSE, only in the vegetative virus
Method to investigate viral P are?
PAGE
Immunobloting (Western Blot)
Immunoperoxidase staining
Monoclonal antibody production
PAGE is a way to investigate……? What do we get to know of them?
Proteins
Number and size of the viral polypeptides
Immunoperoxidase staining detects?
viral antigens
