General characteristics and purification of the viral nucleic acidMethods of nucleic acid investigation I

Question 1

Viral nucleic acid (for the virion)

Answer 1

core

Question 2

Is it big

Answer 2

small, 3-300 kilobase

Question 3

Is it continuous or segmented

Answer 3

yes

Question 4

Is it linear or circular

Answer 4

yes

Question 5

Exception for haploid is?

Answer 5

retrovirus

Question 6

alien NA is contained in?

Answer 6

polyoma, pesti - host cell NA
arena - ribosomes
retro - onc, src genes

Question 7

NA purification

Answer 7

proteinase K E digestion+phenol+chloroform+isoamyloalcohol
or
chromatography

Question 8

which way of extraction is more effective and less dangerous

Answer 8

chromatography

Question 9

+ sense viruses are coded in which direction?

Answer 9

5’-3’

Question 10

• sense viruses are coded in which direction?

Answer 10

3’-5’

Question 11

In electrophoresis information is not proportional to the size?

Answer 11

false, it is

Question 12

restriction endonuclease analysis results in?

Answer 12

sticky and blunt ends made by the E

they can stick together when created by the same E

Question 13

restriction endonuclease is used for?

Answer 13

physical mapping in PCR

Question 14

molecular cloning of DNA

Answer 14

propagation of viral DNA fragments into b.
mass DNA production
protein production (expression plasmids)

Question 15

Blotting (попиване)

Answer 15

Nylon/hybond filter

easier to handle