vibrio, camphylobacter, lawsonia, helicobacter, arcobacter Flashcards

1
Q

vibrio habitat

A
  • water
  • intestine aquatic animals
  • seafood
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2
Q

vibrio morphology

A

small curved rod, one polar flagellum

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3
Q

vibrio gram staining

A

neg

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4
Q

is vibrio fastidious

A

no

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5
Q

vibrio opt temperature

A

lower than 37 degrees

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6
Q

vibrio culture

A
  • fac anaerobes

- selective culture at pH 8.6

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7
Q

vibrio catalase

A

pos

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8
Q

vibrio oxidase

A

pos

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9
Q

vibrio OF

A

fermentative

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10
Q

vibrio pathogenicity

A

pathogens - fac pathogens - saphrphytes

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11
Q

vibrio species

A
  • v cholerae
  • v metschnikovii
  • v parahaemolyticus
  • listonella anguillarum
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12
Q

v cholerae O groups

A

155

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13
Q

v cholerae diseases

A
  • human cholera

- vomiting and profuse watery diarrhoea

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14
Q

biotypes of O1 serotype v cholerae

A

classical and el-tor

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15
Q

O139 serogroup v cholerae

A

v cholerae bengal

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16
Q

v metschnikovii habitat

A
  • water, sewage

- aquatic animals

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17
Q

v metschnikovii oxidase

A

neg

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18
Q

v metschnikovii pathogenicity

A

poultry haemorrhagic enteritis

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19
Q

v parahaemolyticus habitat

A

sea animals

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20
Q

v parahaemolyticus virulance factor

A

thermostable haemolysin

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21
Q

v parahaemolyticus pathogenicity

A

food borne infection in humans - associated with consumption of raw or undercooked seafood

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22
Q

listonella anguillarum habitat

A

marine fish - skin lesions, septicaemia

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23
Q

camphylobacter habitat

A

mucus membranes, food

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24
Q

camphylobacter morphology

A

medium curved rods, S forms, spiral filaments, one polar flagellum

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25
Q

camphylobacter gram staining

A

neg

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26
Q

is camphylobacter fastidious

A

yes

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27
Q

camphylobacter culture

A
  • blood agar
  • microaerophilic
  • selective culture
  • semi solid media
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28
Q

c fetus 25 degrees

A

yes

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29
Q

c fetus 42 degrees

A

no

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30
Q

c jejuni 25 degrees

A

no

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31
Q

c jejuni 42 degrees

A

yes

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32
Q

camphylobacter catalse

A

pos, except for c mucosalis, c sputorum

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33
Q

camphylobacter oxidase

A

pos

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34
Q

does camphylobacter have carbohydrate splitting

A

no

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35
Q

camphylobacter biochemiccal characteristics

A

species, subspecies

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36
Q

camphylobacter antigens

A

cell wall antigens

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37
Q

camphylobacter c fetus

A

c1 and c2

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38
Q

camphylobacter c jejuni/c coli

A

66 serogroups

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39
Q

camphylobacter resistance

A

weak

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40
Q

camphylobacter pathogenicity

A

faculative pathogens - saphrphytes

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41
Q

c fetus ssp fetus disease

A

ovine genital camphylobacteriosis

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42
Q

what does ovine genital camphylobacteriosis cause

A
  • in sheep abortion

- mammals, birds entertitis

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43
Q

c fetus ssp fetus abortion of ewe, liver from fetus

A

round necrotic lesions up to 2cm in diameter with pale raised rims, dark depressed centre on liver surface

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44
Q

c fetus ssp venerealis disease

A

bovine genital camphylobacteriosis

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45
Q

bovine genital bacteriosis causes what

A
  • bulls are asymptomatic carriers - infect cows

- cattle abortion, infertility

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46
Q

c jejuni pathogenicity mammals and birds

A

gut

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47
Q

c jejuni pathogenicity sheep

A

abortion

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48
Q

c jejuni pathogenicity dog and human

A

intestinal camphylobacteriosis

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49
Q

c jejuni pathogenicity birds

A

avian camphylobacter hepatitis

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50
Q

c lari pathogenicity

A

gut, enteritis

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51
Q

c coli pathogenicity swine, human

A

enterocolitis

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52
Q

c mucosalis, c hyointestinalis pathogenicity

A

swine gut, saprophytes

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53
Q

c sputorum pathogenicity

A

saprophytes

54
Q

lawsonia intracellularis habitat

A

swine enterocytes, obligate intracellular

55
Q

lawsonia intracellularis morphology

A

curved thin rods

56
Q

lawsonia intracellularis gram staining

A

neg

57
Q

lawsonia intracellularis culture

A

enterocyte cell culture

58
Q

lawsonia intracellularis pathogenicity

A

swine proliferative enteropathy

59
Q

what does swine proliferative enteropathy cause

A

thickening of the intestinal wall, necrosis of mucusal membrane, enlargement of mesenterial lymph nodes

60
Q

helicobacter habitat

A
  • mammals

- mucus membranes

61
Q

helicobacter morphology

A

small curved helical rods, lophotrich flagella

62
Q

helicobacter gram staining

A

neg

63
Q

helicobacter culture

A
  • microaerophilic

- fastidious

64
Q

helicobacter catalase

A

pos

65
Q

helicobacter oxidase

A

pos

66
Q

helicobacter urease

A

pos

67
Q

does helicobacter split carbohydrates

A

no

68
Q

does helicobacter produce cytotoxins

A

yes, H pylori

69
Q

helicobacter resistance

A

weak

70
Q

h pylori pathogenicity

A

human chronic gastritis, gastric/duodenal ulcer

71
Q

h felis pathogenicity

A

cat, dog

72
Q

h canis species

A

dog

73
Q

h mustelae species

A

weasel

74
Q

h acinonychis species

A

cheetah

75
Q

h bizzozeronii

A

swine gastric ulcer, dog, cat

76
Q

arcobacter habitat

A

mucus membranes, genitals and intestines

77
Q

arcobacter morphology

A

small curved rods, flagella

78
Q

arcobacter gram staining

A

neg

79
Q

arcobacter culture

A
  • microaerophilic - aerotolerant

- leptospira and camphylobacter-media

80
Q

a cryaerophilus pathogenicity cattle, swine

A

abortion, mastitis, faeces

81
Q

a skirrowii pathogenicity cow, pig, sheep

A

abortion, prepuce of bull, faeces

82
Q

a butzleri pathogenicity human, apes

A

enteritis

83
Q

a butzleri pathogenicity cattle

A

abortion

84
Q

spirochaetes habitat

A

mucus membranes, arthropods, environment

85
Q

spirochaetes morphology

A

big thick helical filaments, no classical flagella

86
Q

spirochaetes gram staining

A

neg

87
Q

spirochaetes giemsa staining

A

with fixation with methanol, methylene-blue and eosin and water

88
Q

spirochaetes india ink staining

A

yes

89
Q

spirochaetes levaditis silver impregnation staining

A

yes

90
Q

genera of spirochaetes

A
  • borrelia
  • treponema
  • brachyspira
  • leptospira
91
Q

borrelia, treponema staining

A

difficult or not possible, animal trial

92
Q

brachyspira, leptospira staining

A

can be cultured, special demands

93
Q

borrelia habitat

A

infected animals, rodents, arthropods, tropical countries

94
Q

borrelia morphology

A

big, periplasmic flagella, irregular

linear chromosone

95
Q

borrelia species

A
b anserina
b theileri
b burgdorferi
b afzelii
b garinii
b recurrentis
b hispanica
b caucasica
96
Q

b anserina staining

A

fuchsin, giemsa, levaditis silver impregnation

unstained

97
Q

b anserina culture

A

fastidious, embryonated egg, animal trial

98
Q

b anserina resistance

A

weak

99
Q

b anserina pathogenicity

A

avian borreliosis, hen, turkey, pheasant, goose, duck

100
Q

b burgdorferi, b afzelii, b garinii staining

A

giemsa, unstained

101
Q

b burgdorferi, b afzelii, b garinii culture

A

difficult, fastidious

102
Q

b burgdorferi, b afzelii, b garinii pathogenicity

A

natural hosts - small rodents, ticks

lyme disease- humans, dog, horse

103
Q

treponema habitat

A

infected aniamsl, humans, mucus membranes of oral cavity adn genital

104
Q

treponema staining

A

pathogenic species cannot be stained, or staining is poor

105
Q

treponema culture

A

pathogenic species cannot be cultured

106
Q

treponema species

A

t pallidum, t paraluiscuniculi, t denticola, t parvum

107
Q

t pallidum disease

A

human syphilis

108
Q

t paraluiscuniculi disease

A

rabbit syphilis

109
Q

brachyspira habitat

A

swine large intestine

110
Q

brachyspira morphology

A

medium helical rods

111
Q

brachyspira staining

A

fuchsin, giemsa, unstained

112
Q

brachyspira culture

A

obligate anaerobic, fastidious

113
Q

b hyodysenteriae culture is it fastidious

A

yes

114
Q

b hyodysenteriae selective isolation

A

spectinomycin

115
Q

b hyodysenteriae culture

A

obl anaerobic, blood agar 3-7 days, beta-haemolysis

116
Q

b hyodysenteriae antigens

A

9 serogroups

117
Q

b hyodysenteriae resistance

A

weak, 1 week in faeces

118
Q

b hyodysenteriae pathogenicity

A

fac pathogenic, swine dysentery

119
Q

b pilosicoli

A

weak haemolysis, swine intestinal spirochaetosis

120
Q

b intermedia disease

A

swine spirochaeta colitis

121
Q

b intermedia haemolysis

A

weak

122
Q

b innocens features

A

saprophyte, weak haemolysis, biochemical differences

123
Q

leptospira habitat

A

infected animals, environment, water

124
Q

leptospira morphology

A

big helical rods, hooked

125
Q

leptospira staining

A

unstained, levaditis silver impregnation

126
Q

leptospira culture

A

aerobic, liquid or semisolid, nutrients, korthof medium

127
Q

leptospira what kind of nutrients are needed for culture

A

free fatty acids, inorganic N, vitamins B1 and B12

128
Q

what kind of lipase production is characteristic for leptospira

A

extracellular

129
Q

leptospira resistance

A

weak, 60 degrees minutes, dry enviro hours, natural waters weeks

130
Q

leptospira species

A
65 ssp
l interrogans
l borgpetersenii
l kirschneri
l biflexa
l weilii