L+ enterobacteria Flashcards
1
Q
habitat
A
gut, environment, normal gut flora
2
Q
morphology
A
1-3micrometer rods, capsule, fimbria, flagella
3
Q
shigella and klebsiella are exceptions because they lack what
A
flagella
4
Q
staining
A
gram-
5
Q
culture
A
- simple, faculative anaerobic, 10-45degrees (opt 37)
- selective and differential
- selective enrichment -> salmonella
- combined media -
6
Q
advantage of using combined media
A
recieve more than one kind of feature of bacterial stain
7
Q
cell wall features
A
- peptidoglycan layer is thin
- outer membrane with lipopolysaccharide
- you can remove the blue colour bc of peptidoglycan layer
8
Q
culture
A
selective and differential media
9
Q
types of selective and differential media
A
- low selective capacity
- medium
- high
10
Q
low selective capacity media will seperate what
A
all enterobacteria and other Gr-
11
Q
low selective capacity media
A
-crystal violet - lactose - bromo-thymol-blue/litmus/drigalski
, crystal violet-bile salts - lactose - neutral red/MacConkey
12
Q
medium capacity media will seperate what
A
salmonella and E coli
13
Q
medium capacity media examples
A
-brilliant green - lactose - phenol red
,deoxycholate-citrate - lactose - neutral red/DC
14
Q
high selective capacity will seperate what
A
only salmonella
15
Q
high selective capacity example
A
bismuth-sulphite agar
16
Q
colour of salmonella colonies on bismuth agar
A
black
17
Q
biochemistry
A
catalase +
oxidase -
fermentative
18
Q
cell wall antigens, o
A
lipopolysaccharide
19
Q
capsule antigens, k
A
polysaccharide
20
Q
flagella antigens, h
A
protein
21
Q
fimbria antigens, f
A
protein
22
Q
resistance
A
fair
23
Q
pathogenicity
A
- obligate pathogenic
- faculative pathogenic
- saprophytes - majority
24
Q
genus grouping
A
biochemical characteristics
25
species grouping
biochemical and serological characteristics
26
serogroups grouping
o antigens
27
serotype grouping
o-k-h-f antigens
28
lactose positive genera
- escherichia
- klebsiella
- enterobacter
29
lactose negative genera
-salmonella
-shigella
yersinia
-proteus
-providencia
-edwardsiella
-serratia
-citrobacter
30
e coli habitat
gut, aerobic, faculative anaerobic glut flora
31
e coli morphology
2-3micrometer rods, capsule, flagella, fimbria
32
e coli staining
Gr-
33
describe e coli under gram staining
- middle sized
- rod shaped
- pink
34
e coli culture
- capsulated - calf origin
| - haemolytic - swine origin
35
generation time e coli
20mins
36
ways to differentiate between saprophytic and pathogenic e coli
only pathogenic calf has capsule and only pathogenic swine haemolyses
37
e coli indol production
+
38
e coli methy red test
+
39
e coli voges-proskauer
-
40
e coli citrate utilisation
-
41
e coli cell antigens, o
174 types
42
e coli capsule antigens, k
heat stable and heat labile - 80 types
43
e coli fimbria antigens, f
19 types - virulence factor
44
f1
common fimbria
45
f2,3
human
46
f4,6,18 ab/ac
pig
47
f5,17,41
calves
48
f5
lamb
49
f7-16
urinary tract infection human and poultry
50
e coli flagella antigens, h
57 types
51
e coli resistance
medium
52
types of pathogenic e coli strains
- enteropathogenic or attaching/effacing
- enterotoxigenic strains
- verotoxigenic
- necrotoxic
- enteroinvasive strains
- enteroadhesive-aggregative strains
- enterohaemorrhagic strains
- septicaemic strians
53
e coli diseases in cattle
- calf coli diarrhoea
- calf coli septicaemia
- cow mastitis
54
e coli diseases in swine
- coli-diarrhoea of neonatal pigs
- weaned piglets; coli diarrhoea, oedema disease
- sow mastitis, metritis
55
e coli diseases in lamb and kids
coli diarrhoea
56
e coli diseases in rabbits
diarrhoea
57
e coli disease in poultry
- day old chicken septicaemia
- chicken septicaemia, resp disease
- older - lesions in organs
58
e coli disease in foal, dog, cat, mink, fox, nutria, chincilla
coli diarrhoea
| septicaemia
59
e coli human diseases
```
neonatal enteritis
septicaemia
urogenital infections
enteritis
haemorrhagic enterocolitis
```
60
enteropathogenic e coli or attaching/effacing
- intimin needed for colonisation
- destruction of microvilli, atrophy and shedding of enterocytes
- maldigestion, malabsorption
- diarrhoea in piglets, lambs and pups
61
enterotoxigenic e coli
- adhesins
- enterotoxins
- diarrhoea in neonatal piglets, lambs and calves
- post weaning diarrhoea in pigs
62
enterotoxigenic e coli adhesins
f4 - pigs
| -f5 calves and lambs
63
enterotoxigenic e coli enterotoxins
- LT - induce hypersecretion - heat labile
| - ST - reduces absorption - heat stable
64
verotoxigenic e coli
- bind to erythrocytes
- verotoxins
- damage to vasculature in intestine and other locations
- oedema diseases in weaned piglets
- haemorrhagic enterocolitis in calves
- post weaning diarrhoea in pigs
- haemorrhagic colitis
65
verotoxigenic e coli verotoxins
VT1, vt2, vt2e
66
neurotoxigenic e coli
-bind to erythrocytes
-cytotoxic necotising factors
damage to erythrocytes
67
neurotoxigenic e coli diseases
- haemorrhagic colitis in cattle
- enterocolitis in piglets and calves
- diarrhoea in rabbits
- dysentery in horses
68
septicaemia e coli
- invasion of blood streams
| - endotoxin effect
69
septicaemic e coli diseases
- colisepticaemia in calves, piglets, pups and chickens
- watery mouth in lambs
- arthritis, meningitis
70
uropathogenic e coli
- adhesins needed for colonisation
| - endotoxins and exotoxins - local reactions
71
uropathogenic e coli diseases
cystitis in bitches
72
oportunistic e coli strains diseases
- mastitis in cattle and sows
- pyometra in bitches and queens
- metritis
- omphalitis in calves, lambs and chicks
73
whats omphalitis
inflammation of umbilical cord
74
klebsiella habitat
gut, resp tract, genital tract, environment
75
klebsiella morphology
rod, capsule. no flagella
76
klebsiella culture
- large, merging mucoid colonies
| - rapid change of colour of indicator
77
klebsiella indol production
-
78
klebsiella methy red reaction
-
79
kblla voges-proskauer
+
80
kblla citrate utilisation
+
81
kblla antigens
82 serogroups
82
kblla pathogenicity
saprophytic - faculative pathogenic
83
kblla disease calf
bronchitis, bronchopneumonia
84
kblla disease cow
mastitis
85
kblla disease mare
metritis
86
kblla disease dog
UTI
87
kblla disease human
pneumonia, urogenital infections
88
enterobacter habitat
gut, environment
89
enterobacter morphology
rod, capsule, flagella
90
enterobact culture
mucoid colonies
91
enterobacter indol production
-
92
enterobacter methyl red
-
93
enterobact voges-proskauer
+
94
enterobact citrate utilisation
+
95
enterobact pathogenicity
saprophytic, rarely resp infection in humans
96
enterobact species
- cloacae
| - aerogenes
97
major enteric and systemic pathogens
- e coli
- salmonella
- yersinia ssp
- shigella ssp
98
opportunistic pathogens
- klebsiella sp
- enterobacter sp
- proteus sp
- serratia sp
