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Bacto > L+ enterobacteria > Flashcards

L+ enterobacteria Flashcards

1
Q

habitat

A

gut, environment, normal gut flora

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2
Q

morphology

A

1-3micrometer rods, capsule, fimbria, flagella

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3
Q

shigella and klebsiella are exceptions because they lack what

A

flagella

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4
Q

staining

A

gram-

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5
Q

culture

A
  • simple, faculative anaerobic, 10-45degrees (opt 37)
  • selective and differential
  • selective enrichment -> salmonella
  • combined media -
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6
Q

advantage of using combined media

A

recieve more than one kind of feature of bacterial stain

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7
Q

cell wall features

A
  • peptidoglycan layer is thin
  • outer membrane with lipopolysaccharide
  • you can remove the blue colour bc of peptidoglycan layer
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8
Q

culture

A

selective and differential media

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9
Q

types of selective and differential media

A
  • low selective capacity
  • medium
  • high
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10
Q

low selective capacity media will seperate what

A

all enterobacteria and other Gr-

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11
Q

low selective capacity media

A

-crystal violet - lactose - bromo-thymol-blue/litmus/drigalski
, crystal violet-bile salts - lactose - neutral red/MacConkey

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12
Q

medium capacity media will seperate what

A

salmonella and E coli

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13
Q

medium capacity media examples

A

-brilliant green - lactose - phenol red

,deoxycholate-citrate - lactose - neutral red/DC

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14
Q

high selective capacity will seperate what

A

only salmonella

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15
Q

high selective capacity example

A

bismuth-sulphite agar

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16
Q

colour of salmonella colonies on bismuth agar

A

black

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17
Q

biochemistry

A

catalase +
oxidase -
fermentative

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18
Q

cell wall antigens, o

A

lipopolysaccharide

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19
Q

capsule antigens, k

A

polysaccharide

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20
Q

flagella antigens, h

A

protein

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21
Q

fimbria antigens, f

A

protein

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22
Q

resistance

A

fair

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23
Q

pathogenicity

A
  • obligate pathogenic
  • faculative pathogenic
  • saprophytes - majority
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24
Q

genus grouping

A

biochemical characteristics

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25
Q

species grouping

A

biochemical and serological characteristics

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26
Q

serogroups grouping

A

o antigens

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27
Q

serotype grouping

A

o-k-h-f antigens

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28
Q

lactose positive genera

A
  • escherichia
  • klebsiella
  • enterobacter
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29
Q

lactose negative genera

A

-salmonella
-shigella
yersinia
-proteus
-providencia
-edwardsiella
-serratia
-citrobacter

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30
Q

e coli habitat

A

gut, aerobic, faculative anaerobic glut flora

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31
Q

e coli morphology

A

2-3micrometer rods, capsule, flagella, fimbria

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32
Q

e coli staining

A

Gr-

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33
Q

describe e coli under gram staining

A
  • middle sized
  • rod shaped
  • pink
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34
Q

e coli culture

A
  • capsulated - calf origin

- haemolytic - swine origin

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35
Q

generation time e coli

A

20mins

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36
Q

ways to differentiate between saprophytic and pathogenic e coli

A

only pathogenic calf has capsule and only pathogenic swine haemolyses

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37
Q

e coli indol production

A

+

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38
Q

e coli methy red test

A

+

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39
Q

e coli voges-proskauer

A

-

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40
Q

e coli citrate utilisation

A

-

41
Q

e coli cell antigens, o

A

174 types

42
Q

e coli capsule antigens, k

A

heat stable and heat labile - 80 types

43
Q

e coli fimbria antigens, f

A

19 types - virulence factor

44
Q

f1

A

common fimbria

45
Q

f2,3

A

human

46
Q

f4,6,18 ab/ac

A

pig

47
Q

f5,17,41

A

calves

48
Q

f5

A

lamb

49
Q

f7-16

A

urinary tract infection human and poultry

50
Q

e coli flagella antigens, h

A

57 types

51
Q

e coli resistance

A

medium

52
Q

types of pathogenic e coli strains

A
  • enteropathogenic or attaching/effacing
  • enterotoxigenic strains
  • verotoxigenic
  • necrotoxic
  • enteroinvasive strains
  • enteroadhesive-aggregative strains
  • enterohaemorrhagic strains
  • septicaemic strians
53
Q

e coli diseases in cattle

A
  • calf coli diarrhoea
  • calf coli septicaemia
  • cow mastitis
54
Q

e coli diseases in swine

A
  • coli-diarrhoea of neonatal pigs
  • weaned piglets; coli diarrhoea, oedema disease
  • sow mastitis, metritis
55
Q

e coli diseases in lamb and kids

A

coli diarrhoea

56
Q

e coli diseases in rabbits

A

diarrhoea

57
Q

e coli disease in poultry

A
  • day old chicken septicaemia
  • chicken septicaemia, resp disease
  • older - lesions in organs
58
Q

e coli disease in foal, dog, cat, mink, fox, nutria, chincilla

A

coli diarrhoea

septicaemia

59
Q

e coli human diseases

A 
neonatal enteritis
septicaemia
urogenital infections
enteritis
haemorrhagic enterocolitis
60
Q

enteropathogenic e coli or attaching/effacing

A
  • intimin needed for colonisation
  • destruction of microvilli, atrophy and shedding of enterocytes
  • maldigestion, malabsorption
  • diarrhoea in piglets, lambs and pups
61
Q

enterotoxigenic e coli

A
  • adhesins
  • enterotoxins
  • diarrhoea in neonatal piglets, lambs and calves
  • post weaning diarrhoea in pigs
62
Q

enterotoxigenic e coli adhesins

A

f4 - pigs

-f5 calves and lambs

63
Q

enterotoxigenic e coli enterotoxins

A
  • LT - induce hypersecretion - heat labile

- ST - reduces absorption - heat stable

64
Q

verotoxigenic e coli

A
  • bind to erythrocytes
  • verotoxins
  • damage to vasculature in intestine and other locations
  • oedema diseases in weaned piglets
  • haemorrhagic enterocolitis in calves
  • post weaning diarrhoea in pigs
  • haemorrhagic colitis
65
Q

verotoxigenic e coli verotoxins

A

VT1, vt2, vt2e

66
Q

neurotoxigenic e coli

A

-bind to erythrocytes
-cytotoxic necotising factors
damage to erythrocytes

67
Q

neurotoxigenic e coli diseases

A
  • haemorrhagic colitis in cattle
  • enterocolitis in piglets and calves
  • diarrhoea in rabbits
  • dysentery in horses
68
Q

septicaemia e coli

A
  • invasion of blood streams

- endotoxin effect

69
Q

septicaemic e coli diseases

A
  • colisepticaemia in calves, piglets, pups and chickens
  • watery mouth in lambs
  • arthritis, meningitis
70
Q

uropathogenic e coli

A
  • adhesins needed for colonisation

- endotoxins and exotoxins - local reactions

71
Q

uropathogenic e coli diseases

A

cystitis in bitches

72
Q

oportunistic e coli strains diseases

A
  • mastitis in cattle and sows
  • pyometra in bitches and queens
  • metritis
  • omphalitis in calves, lambs and chicks
73
Q

whats omphalitis

A

inflammation of umbilical cord

74
Q

klebsiella habitat

A

gut, resp tract, genital tract, environment

75
Q

klebsiella morphology

A

rod, capsule. no flagella

76
Q

klebsiella culture

A
  • large, merging mucoid colonies

- rapid change of colour of indicator

77
Q

klebsiella indol production

A

-

78
Q

klebsiella methy red reaction

A

-

79
Q

kblla voges-proskauer

A

+

80
Q

kblla citrate utilisation

A

+

81
Q

kblla antigens

A

82 serogroups

82
Q

kblla pathogenicity

A

saprophytic - faculative pathogenic

83
Q

kblla disease calf

A

bronchitis, bronchopneumonia

84
Q

kblla disease cow

A

mastitis

85
Q

kblla disease mare

A

metritis

86
Q

kblla disease dog

A

UTI

87
Q

kblla disease human

A

pneumonia, urogenital infections

88
Q

enterobacter habitat

A

gut, environment

89
Q

enterobacter morphology

A

rod, capsule, flagella

90
Q

enterobact culture

A

mucoid colonies

91
Q

enterobacter indol production

A

-

92
Q

enterobacter methyl red

A

-

93
Q

enterobact voges-proskauer

A

+

94
Q

enterobact citrate utilisation

A

+

95
Q

enterobact pathogenicity

A

saprophytic, rarely resp infection in humans

96
Q

enterobact species

A
  • cloacae

- aerogenes

97
Q

major enteric and systemic pathogens

A
  • e coli
  • salmonella
  • yersinia ssp
  • shigella ssp
98
Q

opportunistic pathogens

A
  • klebsiella sp
  • enterobacter sp
  • proteus sp
  • serratia sp

Bacto (19 decks)

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