metabolism Flashcards

1
Q

whats metabolism

A

the sum of biochemical reactions required for energy generation and the use of energy to synthesise cell material from small molecules

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2
Q

two types of metabolism os bacteria

A

catabolic

anabolic

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3
Q

possible carbon sources for bact

A

glucose, lactose, pyruvic acid

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4
Q

possible nitrogen sources for bact

A

protein hydrolysates, peptone, tryptone

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5
Q

nutrient demand of bact

A

-carbon
-nitrogen
-phosphates
-sulphates
-potassium
-magnesium
-calcium
-iron
vitamins
additives

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6
Q

two types of metabolic enzymes of bact

A

intracellular

extracellular

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7
Q

movement of nutrients into the cell of a bact

A

through cell wall, cytoplasmic membrane, porins, permeases - they need a constant energy supply

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8
Q

two main groups of bact based on metabolism

A

autotrophic

heterotrophic

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9
Q

are inorganic materials enough for autotrophic bacteria

A

yes

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10
Q

c source for autotrophic bact

A

CO2

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11
Q

n source for autroph bact

A

N2, NH3, NH4 salts

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12
Q

two types of autotroph bact

A

photoautotoph

chemoautotroph

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13
Q

photoautotrop bact features

A

photosynthesis

H donor

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14
Q

chemoautotroph bact features

A

oxidation of inorganic materials
nitrification bact
saprophytes

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15
Q

what kind of c sources are needed for heterotoph bact

A

organic c sources - carbohydrates, alcohols, amino acids

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16
Q

N demand of heteroph bact

A

inorganic N or amino acids or proteins

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17
Q

are lipids needed by heterotroph bact

A

no, except leptospira

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18
Q

what type of lipid does leptospira need

A

free fatty acids

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19
Q

paratrophic bact are a type of what

A

heterotoph bact

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20
Q

paratrophic bact features

A

no propagation on artificial media, obligate intracellular bact

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21
Q

pathogenicity of heterotoph bact

A

saprophytes and pathogens

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22
Q

energy yielding mechanisms in eukaryotes

A

alcoholic fermentation
lactic acid fermentation
aerobic respiration
photosynthesis

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23
Q

whats anaerobic respiration

A

respiration that uses substances other than O2 as a final electron acceptor

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24
Q

whats lithotrophy

A

use of inorganic substance as sources of energy

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25
Q

what photoheterotrophy

A

use of organic compounds as a carbon source during bacterial photosynthesis

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26
Q

anoxygenic photosynthesis what is it

A

photphosphorylation in the absence of O2

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27
Q

whats methogenesis

A

uses H2 as an energy source and produces methane

28
Q

whats light driven non photosynthetic photophosphorylation

A

unique archaen metabolism that converts light energy into chemical energy

29
Q

how do e coli produce energy for growth

A

by respiration or fermentation

30
Q

how can e coli respire aerobically

A

using O2 as a final electron acceptor

31
Q

how can e coli respire anaerobically

A

using NO3 or fumarate as a terminal electron acceptor

32
Q

what is produced in respiration (oxidation)

A

H2O2 and energy

33
Q

whats fermentation

A

oxidation with dehydrogenation

34
Q

in which form is the excess carbohydrate produced in fermentation

A

lactic acid

35
Q

obligate anaerobic bact features

A
  • propagation in low redox potential media
  • fermentation products are characteristic
  • cytochrome system is missing
  • catalase and peroxidase are missing
36
Q

anaerobic bact genera

A
clostridium
actinomyces
fusobacterium
bacteroides
brachyspira
37
Q

energy production in obligate aerobic bact

A

energy prod with bact

38
Q

aerobic bact genera

A
bacillus
micrococcus
mycobacterium
nocardia
brucella
pseudomonas, burkholderia
bordetella
moraxella
39
Q

growth of fac anaerobic bact

A

in presence or absence of O2

40
Q

energy production in fac anaerobic bact

A

oxidation

fermentation

41
Q

which bact need protein

A

trueperella pyogenes

42
Q

which bact need amino acids

A

peptone, tryptone

43
Q

which bact need inorganic nitrogne demand

A

autotrophs

44
Q

bacteria utilising N2

A

azotobacter, clostridium, rhizobium, rumen of ruminants

45
Q

bacteria using ammonium salts and ammonia

A

used directly to amino acid synthesis, nitrification bact, ruminants

46
Q

reactions done by bact using amino acids and other nitrogen compounds

A

decarboxylation

deamination

47
Q

products produced by bact using AAs and other N compounds

A

amines, keto acids, acetic acid, lactic acid, butyric acid, valeric acid, NH3, H2, CO2, H2O, H2S, indole

48
Q

two types of proteolytic bact

A

aerobic

anaerobic

49
Q

example of aerobic proteolytic bact

A

proteus spp

50
Q

anerobic proteolytic bact

A

clostridium botulinum

51
Q

products of proteolytic bact

A

amino acids and diff end products

52
Q

role of bact in N cycle

A

decomposition of dead animals and plants
end product of decomposition NH3, N2
production of NO2, NO3
NO2, NO3 utilised by plants

53
Q

name enzyme that some bact can produce

A

lipase

54
Q

bact that need vitB

A

brucella, lactobacilli - cheese production

55
Q

additives that bact might demand

A

NAD, haemin, PABA, mycobactin, DNA hydrolysate, cysteine

56
Q

NAD needing species

A

haemophilus, avibacterium, glasserella, actinobacillus

57
Q

species that need haemin

A

haemophilus

58
Q

bact that need PABA

A

erysipelothrix rhusiopathiae

59
Q

bact that need mycobactin

A

M avium ssp paratuberculosis

60
Q

bact that need DNA hydrolysate

A

mycoplasma spp

61
Q

bact that need cysteine

A

francisella tularenesis, renibacterium salmonarium

62
Q

reasons for bact to produce pigments

A
in the cell or diffuse to medium
secondary metabolites
material
protect from light, redox processes
environmental effects can influence
63
Q

carbohydrate metabolism tests

A
  • utilisation - acid and gas production
  • citrate utilisation
  • c source utilisation
  • methyl-red test
  • voges-proskauer test
  • OF
  • oxidase
  • catalase
64
Q

tests for n metabolism

A
  • nitrate reduction
  • urease production
  • phenylalanine-deaminase
  • H2S production
  • indol production
  • lysine decarboxylase
65
Q

extracellular enzymes

A
coagulase
gelatinase
proteolytic enzymes
lecitinase
lipase
phosphatase
66
Q

utilisation of bacterial activity

A
decomposition of organic materials
handling of manure
handling of sewage water
self cleaning of natural water
silage production
pickling
dairy productions
fermentation industry
production of enzymes
prod of hormones
AB production
biogas production
microbial insecticides
paint digesting
bioremedation
biomining
desulphurisation of coal