L- enterobacteria Flashcards
1
Q
salmonella taxonomy
A
- 1 species S enterica
- 6 subspecies, mmore than 200 serotypes
2
Q
habitat salmonella
A
gut, sewage, water, feed, food
3
Q
morphology salmonella
A
1-5micrometer rods
-flagella except gallinarum and pullorum
4
Q
salmonella staining
A
Gr-
5
Q
salmonella culture
A
- selective enrichment
- low, medium or high selective capacity
6
Q
selective enrichment salmonella culture
A
- Na-selenite, cystine
- Na-tetrathionate, bile salts
7
Q
salmonella culture colour on drigalski agar
A
blue
8
Q
salmonella culture colour on bismuth sulphite agar
A
black
9
Q
salmonella culture colour on MacConkey agar
A
colourless
10
Q
salmonella culture colour on Rambach agar
A
red
11
Q
salmonella indol production
A
-
12
Q
salmonella methyl red
A
+
13
Q
salmonella voges proskauer
A
-
14
Q
salmonella citrate utilisation
A
+
15
Q
salmonella urease production
A
-
16
Q
salmonella H2S production
A
+
17
Q
salmonella phenylalanine-desaminase prod
A
-
18
Q
salmonella antigens
A
kaufmann white scheme
19
Q
salmonella O antigens
A
51 O groups, composite antigens
20
Q
salmonella K antigens
A
we call it Vi bc theres a strong correlation btwn virulence and capsule antigens
21
Q
salmonella H antigens
A
- specific, phase 1
- non specific phase 2
22
Q
salmonella serotypes
A
- monophasic - only specific
- diphasic - not specific
23
Q
salmonella resistance
A
fair but can survive in damp shaded soil for up to 9months
24
Q
salmonella pathogenicity
A
pathogenic - faculative pathogenic
25
salmonella pathogenic human types
- S typhi - typhoid fever of human
| - S parathyphi A,B,C S sendai - paratyphoid fever
26
salmonella pathogenic animal types
- S typhisuis - swine typhus
- S pullorum - pullorum disease
- S gallinarum - fowl typhoid
27
salmonella pathogenic human-animal types
- S enteridis
- S typhimurium
- S thompson
- S choleraesuis
28
salmonella pathogenic abortion types
- S abortusequi
- S abortusovis
- S abortusbovis
29
citrobacter habitat
mammals, birds, gut, water environment
30
citrobacter biochemistry
- growth on medium containing KCN +
| - lysine decarboxylase activity -
31
pathogenicity citrobacter
saprophytic, sometimes secondary infections
32
citrobacter indol production
-
33
citrobacter methyl red
+
34
cirtobacter voges-prokauer
-
35
citrobacter citrate utilisation
+
36
citrobacter urease
-
37
cirtobacter H2S prod
+
38
citrobacter phenylalanine-desaminase prod
-
39
shigella habitat
intestine, sewage
40
shigella morphology
no flagella
41
shigella culture
Deoxycholate-citrate-lactose-neutral red
42
shigella biochemistry
resembles E coli
43
only biochem test that shigella give positive result
methyl red
44
pathogenic shigella species
- dysenteriae
- flexneri
- sonnei
- boydii
45
human disease caused by shigella sp
bacillary dysentery/shigellosis
46
proteus, providencia habitat
intestine, water, rotting materials
47
proteus, providencia staining
Gr-
48
proteus, providencia culture
swarming - extremely motile, can move on surface of solid medium
49
proteus phenylalanine-desaminase production
+
50
proteus, providencia pathogenicity
saprophytic - they can contaminate urinary tract bc motile
51
yersinia sp
- pestis
- pseudotuberculosis
- enterocolitica
- ruckeri
52
do Y pestis have flagella
no
53
Y pseudotuberculosis habitat
intestine, water
54
Y pseudotuberculosis morphology
1-2micrometer, coccoid rods, flagella 22 degrees
55
Y pseudotuberculosis staining
- Gr-
| - bipolar staining
56
Y pseudotuberculosis culture
- opt temperature 30 degrees, simple, 4-42 degrees small colonies
- selective culture, low selective capacity
57
Y pseudotuberculosis antigens
- 6 o serotypes
- 5 h antigens
- composite antigens
58
Y pseudotuberculosis pathogenicity
- rodents
- turkey, pheasant, quail - septicaemia, abscesses
- cattle, swine, sheep - abortion
- human -- enterocolitis, mesenteric lymph nodes, abscesses, inflammation, enteritis
59
Y enterocolitica habitat
intestine, water
60
Y enterocolitica culture
- opt temp 22
| - selective culture Na-oxalate + bile salts
61
Y enterocolitica antigens
- 34 o antigens, o9 cross reaction with brucella sp
| - 20h antigens
62
Y enterocolitica pathogenicity
- human -GI infection, abscesses
| - swine, rabbit - intestine, mesenteric lymph nodes
63
Y ruckeri biochem
inactive
64
Y ruckeri pathogenicity
trout septicaemia
kidney
-red mouth disease
65
serratia habitat
soil, water
66
serratia culture
lower opt temp, pigment
67
serratia pathogenicity
- saprophytes
| - S marcescens - food contamination
68
edwardsiella habitat
intestine, water
69
edwardsiella culture
fasidious - vitamins, amino acids needed
70
edwardsiella pathogenicity
- E tarda -eel
| - E ictaluri - septicaemia, ascites
71
Gr- anaerobic rods habitat
mucous membranes of Gi tract, genital tract, anaerobic gut flora
72
Gr- anaerobic rods pathogenicity
- faculative pathogens
| - mucouos membranes, wounds, abscesses
73
Gr- anaerobic rods genera
- fusobacterium
- bacteroides
- dichelobacter
74
fusobacterium morphology
polymorph, no flagella
75
fusobacterium staining
Gr-, irregular
76
fusobacterium culture
- fastidious, obligate anaerobic, CO2
| - selective culture; crystal violet, brilliant green, vancomycin
77
fusobacterium biochem
inactive, gelatinase, casein-hydrolysis
78
fusobacterium resistance
weak
79
fusobacterium pathogenicity
faculative pathogenic, anaerobic wounds, abscesses, gangrene
80
fusobacterium species
-F necrophorum ssp necrophorum, funduliforme
81
F necrophorum morphology
long chains, filaments
82
F necrophorum culture
blood agar, obligate anaerobic haemolysis
83
F necrophorum biochemistry
carbohydrates are not utilised, lipase, DN-ase, hyaluronidase
84
F necorphorum spp necrophorum
- haemolysis, haemagluttination, cytotoxin, syngerism
- calf, piglet, rabbit - necrobacillus
- sheep- foot rot
85
F necrophorum spp funduliofrme
no haemagluttination, no cytotoxin, no syngerism
86
F nucleatum
mainly human, dog, cat - abscess, mastitis, metritis, peritonitis, wounds
87
bacteroides and dichelobacter habitat
alimentary tract, genitals, mucous membranes
88
bacteroides and dichelobacter morphology
3-5micrometer rod, fimbria
89
bacteroides + dichelobacter staining
Gr-, irregular
90
bacteroides + dichelobacter culture
fastidious, obligate anaerobic
91
bacteroides + dichelobacter biochem
more active, proteases, keratinase - D nodosus
92
bacteroides + dichelobacter pathogenicity
faculative pathogens, necrosis, abscesses
93
bacteroides + dichelobacter species
- bacteroides fragilis
- bacteroides melaninogenicus
- bacteroides ureolyticus
- dichelobacter nodosus
94
bacteroides fragilis diseases
mainly human, gut flora, appendicitis, wounds
95
bacteroides melaninogenicus diseases
dog, cat pturid inflammations
96
bacteroides ureolyticus
genital tract
97
dichelobacter nodosus
fimbria, proteolysis, keratinase, sheep foot rot
98
three bacterial species that cause foot rot
- fusobacterium necrophorum
- trueperlla pyogenes
- dichelobacter nodosus
