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bacto > L- enterobacteria > Flashcards

L- enterobacteria Flashcards

1
Q

salmonella taxonomy

A
  • 1 species S enterica

- 6 subspecies, mmore than 200 serotypes

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2
Q

habitat salmonella

A

gut, sewage, water, feed, food

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3
Q

morphology salmonella

A

1-5micrometer rods

-flagella except gallinarum and pullorum

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4
Q

salmonella staining

A

Gr-

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5
Q

salmonella culture

A
  • selective enrichment

- low, medium or high selective capacity

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6
Q

selective enrichment salmonella culture

A
  • Na-selenite, cystine

- Na-tetrathionate, bile salts

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7
Q

salmonella culture colour on drigalski agar

A

blue

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8
Q

salmonella culture colour on bismuth sulphite agar

A

black

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9
Q

salmonella culture colour on MacConkey agar

A

colourless

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10
Q

salmonella culture colour on Rambach agar

A

red

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11
Q

salmonella indol production

A

-

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12
Q

salmonella methyl red

A

+

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13
Q

salmonella voges proskauer

A

-

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14
Q

salmonella citrate utilisation

A

+

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15
Q

salmonella urease production

A

-

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16
Q

salmonella H2S production

A

+

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17
Q

salmonella phenylalanine-desaminase prod

A

-

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18
Q

salmonella antigens

A

kaufmann white scheme

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19
Q

salmonella O antigens

A

51 O groups, composite antigens

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20
Q

salmonella K antigens

A

we call it Vi bc theres a strong correlation btwn virulence and capsule antigens

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21
Q

salmonella H antigens

A
  • specific, phase 1

- non specific phase 2

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22
Q

salmonella serotypes

A
  • monophasic - only specific

- diphasic - not specific

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23
Q

salmonella resistance

A

fair but can survive in damp shaded soil for up to 9months

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24
Q

salmonella pathogenicity

A

pathogenic - faculative pathogenic

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25
Q

salmonella pathogenic human types

A
  • S typhi - typhoid fever of human

- S parathyphi A,B,C S sendai - paratyphoid fever

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26
Q

salmonella pathogenic animal types

A
  • S typhisuis - swine typhus
  • S pullorum - pullorum disease
  • S gallinarum - fowl typhoid
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27
Q

salmonella pathogenic human-animal types

A
  • S enteridis
  • S typhimurium
  • S thompson
  • S choleraesuis
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28
Q

salmonella pathogenic abortion types

A
  • S abortusequi
  • S abortusovis
  • S abortusbovis
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29
Q

citrobacter habitat

A

mammals, birds, gut, water environment

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30
Q

citrobacter biochemistry

A
  • growth on medium containing KCN +

- lysine decarboxylase activity -

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31
Q

pathogenicity citrobacter

A

saprophytic, sometimes secondary infections

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32
Q

citrobacter indol production

A

-

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33
Q

citrobacter methyl red

A

+

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34
Q

cirtobacter voges-prokauer

A

-

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35
Q

citrobacter citrate utilisation

A

+

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36
Q

citrobacter urease

A

-

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37
Q

cirtobacter H2S prod

A

+

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38
Q

citrobacter phenylalanine-desaminase prod

A

-

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39
Q

shigella habitat

A

intestine, sewage

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40
Q

shigella morphology

A

no flagella

41
Q

shigella culture

A

Deoxycholate-citrate-lactose-neutral red

42
Q

shigella biochemistry

A

resembles E coli

43
Q

only biochem test that shigella give positive result

A

methyl red

44
Q

pathogenic shigella species

A
  • dysenteriae
  • flexneri
  • sonnei
  • boydii
45
Q

human disease caused by shigella sp

A

bacillary dysentery/shigellosis

46
Q

proteus, providencia habitat

A

intestine, water, rotting materials

47
Q

proteus, providencia staining

A

Gr-

48
Q

proteus, providencia culture

A

swarming - extremely motile, can move on surface of solid medium

49
Q

proteus phenylalanine-desaminase production

A

+

50
Q

proteus, providencia pathogenicity

A

saprophytic - they can contaminate urinary tract bc motile

51
Q

yersinia sp

A
  • pestis
  • pseudotuberculosis
  • enterocolitica
  • ruckeri
52
Q

do Y pestis have flagella

A

no

53
Q

Y pseudotuberculosis habitat

A

intestine, water

54
Q

Y pseudotuberculosis morphology

A

1-2micrometer, coccoid rods, flagella 22 degrees

55
Q

Y pseudotuberculosis staining

A
  • Gr-

- bipolar staining

56
Q

Y pseudotuberculosis culture

A
  • opt temperature 30 degrees, simple, 4-42 degrees small colonies
  • selective culture, low selective capacity
57
Q

Y pseudotuberculosis antigens

A
  • 6 o serotypes
  • 5 h antigens
  • composite antigens
58
Q

Y pseudotuberculosis pathogenicity

A
  • rodents
  • turkey, pheasant, quail - septicaemia, abscesses
  • cattle, swine, sheep - abortion
  • human – enterocolitis, mesenteric lymph nodes, abscesses, inflammation, enteritis
59
Q

Y enterocolitica habitat

A

intestine, water

60
Q

Y enterocolitica culture

A
  • opt temp 22

- selective culture Na-oxalate + bile salts

61
Q

Y enterocolitica antigens

A
  • 34 o antigens, o9 cross reaction with brucella sp

- 20h antigens

62
Q

Y enterocolitica pathogenicity

A
  • human -GI infection, abscesses

- swine, rabbit - intestine, mesenteric lymph nodes

63
Q

Y ruckeri biochem

A

inactive

64
Q

Y ruckeri pathogenicity

A

trout septicaemia
kidney
-red mouth disease

65
Q

serratia habitat

A

soil, water

66
Q

serratia culture

A

lower opt temp, pigment

67
Q

serratia pathogenicity

A
  • saprophytes

- S marcescens - food contamination

68
Q

edwardsiella habitat

A

intestine, water

69
Q

edwardsiella culture

A

fasidious - vitamins, amino acids needed

70
Q

edwardsiella pathogenicity

A
  • E tarda -eel

- E ictaluri - septicaemia, ascites

71
Q

Gr- anaerobic rods habitat

A

mucous membranes of Gi tract, genital tract, anaerobic gut flora

72
Q

Gr- anaerobic rods pathogenicity

A
  • faculative pathogens

- mucouos membranes, wounds, abscesses

73
Q

Gr- anaerobic rods genera

A
  • fusobacterium
  • bacteroides
  • dichelobacter
74
Q

fusobacterium morphology

A

polymorph, no flagella

75
Q

fusobacterium staining

A

Gr-, irregular

76
Q

fusobacterium culture

A
  • fastidious, obligate anaerobic, CO2

- selective culture; crystal violet, brilliant green, vancomycin

77
Q

fusobacterium biochem

A

inactive, gelatinase, casein-hydrolysis

78
Q

fusobacterium resistance

A

weak

79
Q

fusobacterium pathogenicity

A

faculative pathogenic, anaerobic wounds, abscesses, gangrene

80
Q

fusobacterium species

A

-F necrophorum ssp necrophorum, funduliforme

81
Q

F necrophorum morphology

A

long chains, filaments

82
Q

F necrophorum culture

A

blood agar, obligate anaerobic haemolysis

83
Q

F necrophorum biochemistry

A

carbohydrates are not utilised, lipase, DN-ase, hyaluronidase

84
Q

F necorphorum spp necrophorum

A
  • haemolysis, haemagluttination, cytotoxin, syngerism
  • calf, piglet, rabbit - necrobacillus
  • sheep- foot rot
85
Q

F necrophorum spp funduliofrme

A

no haemagluttination, no cytotoxin, no syngerism

86
Q

F nucleatum

A

mainly human, dog, cat - abscess, mastitis, metritis, peritonitis, wounds

87
Q

bacteroides and dichelobacter habitat

A

alimentary tract, genitals, mucous membranes

88
Q

bacteroides and dichelobacter morphology

A

3-5micrometer rod, fimbria

89
Q

bacteroides + dichelobacter staining

A

Gr-, irregular

90
Q

bacteroides + dichelobacter culture

A

fastidious, obligate anaerobic

91
Q

bacteroides + dichelobacter biochem

A

more active, proteases, keratinase - D nodosus

92
Q

bacteroides + dichelobacter pathogenicity

A

faculative pathogens, necrosis, abscesses

93
Q

bacteroides + dichelobacter species

A
  • bacteroides fragilis
  • bacteroides melaninogenicus
  • bacteroides ureolyticus
  • dichelobacter nodosus
94
Q

bacteroides fragilis diseases

A

mainly human, gut flora, appendicitis, wounds

95
Q

bacteroides melaninogenicus diseases

A

dog, cat pturid inflammations

96
Q

bacteroides ureolyticus

A

genital tract

97
Q

dichelobacter nodosus

A

fimbria, proteolysis, keratinase, sheep foot rot

98
Q

three bacterial species that cause foot rot

A
  • fusobacterium necrophorum
  • trueperlla pyogenes
  • dichelobacter nodosus

bacto (19 decks)

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