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Bact211 > VIBRIO > Flashcards

VIBRIO Flashcards

1
Q

Vibrio is commonly found in

A

Fresh water, salt water, and estuarine water

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2
Q

How can you avoid vibrio infection?

A

Avoid eating raw or undercooked shellfish particularly in warmer summer months

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3
Q

What are the most commonly isolated vibrio species?

A
  • vibrio cholerae
  • vibrio parahaemolyticus
  • vibrio vulnificus
  • vibrio alginolyticus
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4
Q

Causes a devastating diarrheal disease cholera

A

Vibrio cholerae

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5
Q

Causes ulcers and has been linked to gastric carcinoma

A

Helicobacter pylori

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6
Q

What are the factors to consider when identifying vibrio infection

A
  • recent consumption of raw seafood
  • recent immigration travel
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7
Q

What species of vibro are catalase positive and oxidase negative?

A

Vibrio metschnikovii

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8
Q

What is the causative agent for cholera?

A

Vibrio cholerae O1

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9
Q

Vibrio cholerae O1 also known as

A

Asiatic cholera

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10
Q

Most common cause of bacterial gastroenteritis

A

Campylobacter jejuni

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11
Q

Aeromonas wound infection almost always present as

A

Cellulitis

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12
Q

A test that is used to differentiate vibrio from members of enterobacteriaceae

A

Oxidase test

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13
Q

Campylobacter fetus is associated with

A

Bacteremia

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14
Q

What is the culture medium of choice for isolation of vibrio species?

A

Thiosulfate Citrate Bile Salt Sucrose

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15
Q

Sucrose ferments in TCBS appears as

A

Yellow

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16
Q

What is the pH indicator in TCBS

A

Bromothymol blue

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17
Q

What is the reagent for string test?

A

Sodium desoxycholate

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18
Q

Selective agar for pseudomonas aeruginosa

A

Cetrimide agar

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19
Q

The concentration of the vibriostatic disc

A

150 ug

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20
Q

What is the atmospheric environment requirement for campylobacter jejuni and what is the incubation temperature?

A

5% oxygen and 10% carbon dioxide at 42 degrees

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21
Q

What causes the formation of mucoid string in string test?

A

DNA

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22
Q

Acute diarrheal disease spread through contaminated water

A

Cholera

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23
Q

Cholera is responsible to what toxin?

A

Cholera toxin or choleragen

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24
Q

What are the two biovar of cholera?

A

Classical type and el tor type

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25
What type of cholera tested positive in voges proskauer?
El tor
26
El Tor is susceptible in which susceptibility testing?
Polymyxin B susceptibility test
27
How do we replace fluids lost from the severe diarrhea?
Oral fluids or intravenous
28
What is the treatment for cholera?
Azithromycin or ciprofloxacin
29
Cholera is resistant to
Tetracycline and doxycycline
30
What is the second most common vibrio implicated in gastroenteritis?
Vibrio parahaemolyticus
31
Vibrio parahaemolyticus is first recognized in which country?
Japan
32
Heat stable hemolysin that is able to lyse human erythrocytes
Kanagawa toxin
33
Special high salt mannitol medium
Wagatsuma agar
34
It is least pathogenic and most frequently isolated vibrio
Vibrio alginolyticus
35
What are the two infections of vibrio vulnificus?
- primary septicemia - wound infection
36
Transport media for vibrio
Cary Blair media
37
What is the color of non sucrose fermenting for vibrio
Green colonies
38
For enrichment procedure what medium is used?
Alkaline peptone water with 1% sodium chloride
39
What is the most commonly isolated specie of aeromonas?
A. caviae
40
Septicemia, meningitid, and wound infections
Extraintestinal infections
41
What is the pigment for aeromonas colonies?
Translucent and white to buff-colored
42
What test is used to differentiate plesiomonas and aeromonas?
Fermentation of inositol
43
What specie of aeromonas is lactose fermenter?
A. caviae
44
What is the mode of transmission for C. Jenuni?
- direct contact with animals and handling infected pets - consumption of water and dairy products - person to person transmission
45
What is the atmospheric environment requirement for helicobacter pylori and what is the incubation temperature?
5-10% oxygen and 5-12% carbon dioxide at 37 degrees
46
What genus plays a role in GBS or Guillan-Barre Syndrome?
Campylobacter spp
47
This genus is classified as carcinogen
Helicobacter spp
48
What is the specimen for c. jejuni?
Stool
49
What is the specimen for c. fetus?
Blood with incubation at 35-37 degrees
50
What is the transport medium for campylobacter?
Cary-Blair medium
51
What is the transport medium for Helicobacter?
Stuart medium
52
What is the specimen for Helicobacter?
Gastric biopsy material
53
What is the selective media for c. jejuni?
Campy-BAP
54
What other media used in c. Jejuni?
Skirrow’s agar and butzler medium
55
What is the commonly used medium in Helicobacter pylori?
CAP or brucella agar with 5% horse blood
56
What is the selective medium for h. pylori?
Skirrow’s agar
57
What is the shape for campylobacter?
Seagull-wing shaped
58
What is the shape for vibrio?
Comma shaped
59
What is the shape for Helicobacter?
Curved shaped or U shaped
60
What is the recommended counter stain for campylobacter?
Carbolfuschin
61
What does campylobacter looks like under phase contrast microscope?
Darting motility or corkscrew movement
62
What is the colony morphology c. fetus?
Smooth, convex, translucent colonies
63
What is the colony morphology for c. jejuni?
Moist, runny looking, and spreading
64
What biochemical test is used to differentiate Helicobacter from campylobacter?
Urease test
65
What test is used to distinguish aeromonas from vibrio?
String test and vibriostatic O/129 Aeromonas String test negative Vibriostatic O/129 resistant Vibrio String test positive Vibriostatic O/129 susceptible
66
Toxin choleragen consists of
Two toxic a subunits and five binding b subunits
67
What are the three test used to differentiate el tor and classical cholera?
Voges proskauer test Polymyxin B susceptibility test Serological test using Chicken RBC as antigen
68
What is the colony morphology of vibrio in SBA?
Medium to large colony ; smoith, opaque, iridescent with greenish hue
69
It is found in soil and aquatic environments Does not tolerate increased sodium chloride
Plesiomonas
70
Widely distributed among warm and cold blooded animals like dogs, cats, lizard, fish, shellfish
Plesiomonas
71
What is the mode of transmission for plesiomonas?
Ingestion of contaminated water or food
72
What is CIN?
Cefsulodin-irgasan novobiocin

Bact211 (10 decks)

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