Specimen Processing Flashcards
Support the growth of most nonfastidious microbes
Nonselective media
Allow grouping of microbes based on different characteristics demonstrated on the medium
Differential media
Can be used as a supplement to agar plates to detect small numbers of most aerobes, anaerobes, and microaerophiles
Broth media
Contain growth enhancers that are added to nonselective agar to allow fastidious organisms to flourish
Enriched media
Support the growth of one type or group of microbes but not another
Selective media
What is the example of nonselective media?
Sheep blood agar
Example of selective media
MacConkey agar
Example of differential media
MacConkey agar and Mannitol Salt Agar
Example of enriched media
Blood agar plate and Chocolate agar plate
Example of enrichment broth
Selenite F, Alkaline Peptone Water, Lim broth
Example of broth media
Thioglycolate broth
Grow with reduced oxygen
Microaerophiles
The procrss preferred for the preparation of thin specimen
Cytocentrifugation
Directed toward coloring the forms and shapes present
Simple stain
Directed toward coloring specific components of the elements present
Differential stain
Directed specifically at identification of an organism
Diagnostic antibody
Process deposits cellulae elements and microorganisms from the specimen onto the surface of a glass slide as a monolayer
Cytocentrifugation
Hydrophobic substance that affects the permeability of the cell well
Mycolic acid
Mycobacteria stain bright orange.
Fluorescent stain
How long wright giemsa stain requires?
1-3 minutes
Fluorochrome dye that stains both gram positive and gram negative bacteria, living or dead
Acridine orange
What is used for scoring sputum
Barlett’s method
Ideal anaerobic incubation. Oxygen free environment
Anaerobic chamber
envelope gas generator. cheaper than anaerobic chamber
Anaerobic jar
