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Bact211 > Culture Media > Flashcards

Culture Media Flashcards

1
Q

Media containung the required nutrients for bacterial growth

A

Culture media

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2
Q

Enumerate the common ingredients of culture media

A
  • Peptone
  • meat extract
  • yeast extract
  • agar
  • water
  • mineral salts
  • carbohydrates
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3
Q

Hydrolyzed product of animal and plants proteins

A

Peptone

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4
Q

Supply amino acids, vitamins and mineral salts

A

Meat extract

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5
Q

Is a bacterial growth stimulants

A

Yeast extract

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6
Q

Phosphates as a source of phosphorus

A

Mineral salts

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7
Q

Simple and complex sugars are a source of a carbon and energy

A

Carbohydrates

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8
Q

Assist in the differentiation of bacteria

A

Carbohydrate

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9
Q

An inert polysaccharide of seaweed

A

Agar

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10
Q

Deionized or distilled water must be used in the preparation of culture media

A

Water

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11
Q

Enumerate the media based on consistency

A

Solid
Semi-solid
Liquid (broth)

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12
Q

Enumerate the media based on nutritional factors

A

Simple (basal)
Complex
Synthetic of define

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13
Q

Enumerate the special media

A

Enriched
Enrichment
Selective
Differential
Transport
Sugar media

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14
Q

Give uses of broth

A

As an enrichment media
As biochemical testing media

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15
Q

Made by adding a solidifying agent to the nutrients and water

A

Solid media

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16
Q

For special purposes where agar is added to media in concentrations that are too low to solidify them

A

Semisolid culture media

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17
Q

Give uses of semi-solid media

A
  • as enrichment media
  • as motility media
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18
Q

Contain only basic nutrients required for the growth of ordinary organisms and used as a general purpose media

A

Simple or basic or all purpose media

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19
Q

Example of simple media

A

Nutrient broth
Nutrient agar

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20
Q

Media that contain some ingredients of unknown chemical composition

A

Complex media

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21
Q

What is the commonly used complex medium

A

Nutrient broth

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22
Q

It is simplest and most common medium ysed routinely in microbiology

A

Nutrient agar

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23
Q

Nutrient agar is commonly referred to as

A

Agar medium

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24
Q

Prepared exclusively from pure chemical substances and their exact composition is known

A

Synthetic or chemically defined media

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25
Q

Prepared to meet the nutritional requirements of more bacteria by the addition of substances such as blood, serum or egg to a basal medium

A

Enriched media

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26
Q

Used for isolation of streptococci, pneumococci, and haemophilus

A

Blood agar plate

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27
Q

Used for isolation of neisseria and hemophilus

A

Chocolate agar plate

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28
Q

Used for isolation of corynebacterium diphtheriae

A

Loeffler’s serum

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29
Q

Substance added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria

A

Enrichment media

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30
Q

Inhibits coliforms ehilr allowing typhoid-paratyphoif bacilli to grow feely in fecal example

A

Tetrathionate broth

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31
Q

Used for dysentery bacilli. Used for isolation of salmonella

A

Selenite F

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32
Q

Used for vibrio cholerae

A

Alkaline Peptone Water

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33
Q

Used to isolate particular bacteria from specimens ehrte mixed bacterial flora is expectrd

A

Selective media

34
Q

Acts as a selective agent for dysentery bacilli. Isolation of shigellae

A

Desoxycholate citrate agar

35
Q

Used for mycobacteria tuberculosis

A

Lowenstein-Jensen medium

36
Q

Allows the growth of vibrio choletae and inhibits the growth of intestinal organisms

A

Bile salt agar

37
Q

Contain an indicator which changes color when a bacterium grows

A

Differential or indicator media

38
Q

Indicates lactose fermenting property

A

McConkey agar

39
Q

Has substances incorporated in it, enabling it to bring out differing characteristics of bacteria and thus helping to distinguish between them

A

Differential media

40
Q

What are the carbohydrates routinely employed for fermentation test

A

Glucose, lactose, sucrose, mannitol

41
Q

Used for test fermentation

A

Sugar media

42
Q

What is color of sugar media

A

Light yellow

43
Q

Acid production us indicated by the development of

A

Pink color

44
Q

Used for characterization and identification of bacteria, particularly important in the study of gram negative bacilli

A

Carbohydrate fermentation

45
Q

Media containing ingredients to prevent the overgrowth of commensals and ensure the survival of pathogenic bacteria when specimens can not be cultured soon after collection

A

Transport media

46
Q

What are the example of transport media

A

Amies transport media
Stuart media
Kelly-Blair media

47
Q

Used to grow anaerobic organisms and contain reducing substancr

A

Anaerobic media

48
Q

Acts as a reducing agent and creates an anaerobic environment deeper in the tube

A

Thioglycollate

49
Q

Acts as an oxidation reduction potential indicator which should show that the medium is anaerobic except in the surfacre layer in addition to a reducing agent and semisolid agar

A

Methylene blue or resazurin

50
Q

What are the methods used to sterilize culture media

A

Autoclaving
Filtration
Steaming to 100•C

51
Q

Used to sterilize most agar and fluid culture media

A

Autoclaving

52
Q

Used to sterilize media containing ingredients that would be inactivated at temperature over 100 degree celcius and re-melt previously bottled sterile agar media

A

Steaming at 100•C

53
Q

Used to sterilize additives that are heat-sensitive and can not be autoclave

A

Filtration

54
Q

What is the simplest eay to test for contamination?

A

Incubate media at 35-37•C for 24 hours

55
Q

What are the uses of culture media?

A
  • isolation and identification of microorganisms
  • performing microbial sensitivity test
56
Q

Give example of agar’s property

A
  • high gelling strength
  • low gelling temperature
  • high melting temperature
57
Q

Example of elevation

A
  • raised
  • flat
  • low convex
  • domed shaped
58
Q

Give example of transparency

A
  • opaque
  • transparent
  • translucent
59
Q

Uses of basic / all purpose media

A
  • To prepare enriched media
  • To maintain stock cultures of control bacterial strains
60
Q

What is the common ingredient of complex media?

A

Peptone

61
Q

A simple basal liquid medium, supports growth of many organisms

A

Nutrient broth

62
Q

What are the types of nutrient broth?

A
  • meat infusion broth
  • meat extract broth
  • digest broth
63
Q

What bacteria can be isolated from BAP?

A
  • streptococci
  • pneumococci
  • haemophilus
64
Q

What bacteria can be isolated from CAP?

A
  • neiserria
  • haemophilus
65
Q

Enumerate the enriched media

A
  • BAP
  • CAP
  • Loeffler’s serum
66
Q

Enumerate the enrichment media

A
  • tetrathionate broth
  • alkaline peptone water
  • selenite f
67
Q

Substance is added to a solid medium which inhibits the growth of unwanted bacteria but favors the growth of wanted bacteria

A

Selective media

68
Q

Enumerate selective media

A
  • desoxycholate citrate agar
  • lowenstein-jensen medium
  • bile salt agar
69
Q

Example of indicator medium

A
  • wilson and blair medium
  • macconkey agar
70
Q

Gas accumulates in the inner Durham’s tube

A

Sugar media

71
Q

Example of Sugar media

A

Hiss serum sugar

72
Q

What is the concentration of agar to prevent convection of air?

A

0.05%

73
Q

Example of anaerobic media

A
  • Thioglycollate broth
  • Robertson’s Cooked Meat Broth
74
Q

Used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms especially clostridium species

A

Roberson’s Cooked Meat broth

75
Q

What is the composition of robertson’s cooked meat broth?

A

Cooked meat of ox heart and nutrient broth

76
Q

General steps in preparation of culture media

A
  1. Weighing and dissolving of culture media ingredients
  2. Sterilization and sterility testing
  3. Addition of heat sensitive ingredients
  4. Dispensing of culture media
  5. pH testing of culture media
  6. Quality assurance of culture media
  7. Storage of culture media
77
Q

What should you do to a refrigerated-heat sensitive ingredients before adding to a molten agar medium?

A

Should be warmed at room temperature

78
Q

What do you do to dehydrated culture media and dry ingredients?

A

Store at an even temperature in a cool dry place

79
Q

What is the required storage temperature for plates of culture media, and additives like serum and blood in SOLID form?

A

2-8 degrees

80
Q

What is the required storage temperature for solution form?

A

-2 degrees

81
Q

It is essentual for the growth of strict anaerobes

A

Anaerobic atmosphere

Bact211 (10 decks)

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