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Bact211 > Culture Media > Flashcards

Culture Media Flashcards

1
Q

Media containung the required nutrients for bacterial growth

A

Culture media

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2
Q

Enumerate the common ingredients of culture media

A
  • Peptone
  • meat extract
  • yeast extract
  • agar
  • water
  • mineral salts
  • carbohydrates
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3
Q

Hydrolyzed product of animal and plants proteins

A

Peptone

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4
Q

Supply amino acids, vitamins and mineral salts

A

Meat extract

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5
Q

Is a bacterial growth stimulants

A

Yeast extract

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6
Q

Phosphates as a source of phosphorus

A

Mineral salts

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7
Q

Simple and complex sugars are a source of a carbon and energy

A

Carbohydrates

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8
Q

Assist in the differentiation of bacteria

A

Carbohydrate

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9
Q

An inert polysaccharide of seaweed

A

Agar

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10
Q

Deionized or distilled water must be used in the preparation of culture media

A

Water

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11
Q

Enumerate the media based on consistency

A

Solid
Semi-solid
Liquid (broth)

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12
Q

Enumerate the media based on nutritional factors

A

Simple (basal)
Complex
Synthetic of define

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13
Q

Enumerate the special media

A

Enriched
Enrichment
Selective
Differential
Transport
Sugar media

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14
Q

Give uses of broth

A

As an enrichment media
As biochemical testing media

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15
Q

Made by adding a solidifying agent to the nutrients and water

A

Solid media

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16
Q

For special purposes where agar is added to media in concentrations that are too low to solidify them

A

Semisolid culture media

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17
Q

Give uses of semi-solid media

A
  • as enrichment media
  • as motility media
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18
Q

Contain only basic nutrients required for the growth of ordinary organisms and used as a general purpose media

A

Simple or basic or all purpose media

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19
Q

Example of simple media

A

Nutrient broth
Nutrient agar

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20
Q

Media that contain some ingredients of unknown chemical composition

A

Complex media

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21
Q

What is the commonly used complex medium

A

Nutrient broth

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22
Q

It is simplest and most common medium ysed routinely in microbiology

A

Nutrient agar

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23
Q

Nutrient agar is commonly referred to as

A

Agar medium

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24
Q

Prepared exclusively from pure chemical substances and their exact composition is known

A

Synthetic or chemically defined media

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25
Prepared to meet the nutritional requirements of more bacteria by the addition of substances such as blood, serum or egg to a basal medium
Enriched media
26
Used for isolation of streptococci, pneumococci, and haemophilus
Blood agar plate
27
Used for isolation of neisseria and hemophilus
Chocolate agar plate
28
Used for isolation of corynebacterium diphtheriae
Loeffler’s serum
29
Substance added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria
Enrichment media
30
Inhibits coliforms ehilr allowing typhoid-paratyphoif bacilli to grow feely in fecal example
Tetrathionate broth
31
Used for dysentery bacilli. Used for isolation of salmonella
Selenite F
32
Used for vibrio cholerae
Alkaline Peptone Water
33
Used to isolate particular bacteria from specimens ehrte mixed bacterial flora is expectrd
Selective media
34
Acts as a selective agent for dysentery bacilli. Isolation of shigellae
Desoxycholate citrate agar
35
Used for mycobacteria tuberculosis
Lowenstein-Jensen medium
36
Allows the growth of vibrio choletae and inhibits the growth of intestinal organisms
Bile salt agar
37
Contain an indicator which changes color when a bacterium grows
Differential or indicator media
38
Indicates lactose fermenting property
McConkey agar
39
Has substances incorporated in it, enabling it to bring out differing characteristics of bacteria and thus helping to distinguish between them
Differential media
40
What are the carbohydrates routinely employed for fermentation test
Glucose, lactose, sucrose, mannitol
41
Used for test fermentation
Sugar media
42
What is color of sugar media
Light yellow
43
Acid production us indicated by the development of
Pink color
44
Used for characterization and identification of bacteria, particularly important in the study of gram negative bacilli
Carbohydrate fermentation
45
Media containing ingredients to prevent the overgrowth of commensals and ensure the survival of pathogenic bacteria when specimens can not be cultured soon after collection
Transport media
46
What are the example of transport media
Amies transport media Stuart media Kelly-Blair media
47
Used to grow anaerobic organisms and contain reducing substancr
Anaerobic media
48
Acts as a reducing agent and creates an anaerobic environment deeper in the tube
Thioglycollate
49
Acts as an oxidation reduction potential indicator which should show that the medium is anaerobic except in the surfacre layer in addition to a reducing agent and semisolid agar
Methylene blue or resazurin
50
What are the methods used to sterilize culture media
Autoclaving Filtration Steaming to 100•C
51
Used to sterilize most agar and fluid culture media
Autoclaving
52
Used to sterilize media containing ingredients that would be inactivated at temperature over 100 degree celcius and re-melt previously bottled sterile agar media
Steaming at 100•C
53
Used to sterilize additives that are heat-sensitive and can not be autoclave
Filtration
54
What is the simplest eay to test for contamination?
Incubate media at 35-37•C for 24 hours
55
What are the uses of culture media?
- isolation and identification of microorganisms - performing microbial sensitivity test
56
Give example of agar’s property
- high gelling strength - low gelling temperature - high melting temperature
57
Example of elevation
- raised - flat - low convex - domed shaped
58
Give example of transparency
- opaque - transparent - translucent
59
Uses of basic / all purpose media
- To prepare enriched media - To maintain stock cultures of control bacterial strains
60
What is the common ingredient of complex media?
Peptone
61
A simple basal liquid medium, supports growth of many organisms
Nutrient broth
62
What are the types of nutrient broth?
- meat infusion broth - meat extract broth - digest broth
63
What bacteria can be isolated from BAP?
- streptococci - pneumococci - haemophilus
64
What bacteria can be isolated from CAP?
- neiserria - haemophilus
65
Enumerate the enriched media
- BAP - CAP - Loeffler’s serum
66
Enumerate the enrichment media
- tetrathionate broth - alkaline peptone water - selenite f
67
Substance is added to a solid medium which inhibits the growth of unwanted bacteria but favors the growth of wanted bacteria
Selective media
68
Enumerate selective media
- desoxycholate citrate agar - lowenstein-jensen medium - bile salt agar
69
Example of indicator medium
- wilson and blair medium - macconkey agar
70
Gas accumulates in the inner Durham’s tube
Sugar media
71
Example of Sugar media
Hiss serum sugar
72
What is the concentration of agar to prevent convection of air?
0.05%
73
Example of anaerobic media
- Thioglycollate broth - Robertson’s Cooked Meat Broth
74
Used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms especially clostridium species
Roberson’s Cooked Meat broth
75
What is the composition of robertson’s cooked meat broth?
Cooked meat of ox heart and nutrient broth
76
General steps in preparation of culture media
1. Weighing and dissolving of culture media ingredients 2. Sterilization and sterility testing 3. Addition of heat sensitive ingredients 4. Dispensing of culture media 5. pH testing of culture media 6. Quality assurance of culture media 7. Storage of culture media
77
What should you do to a refrigerated-heat sensitive ingredients before adding to a molten agar medium?
Should be warmed at room temperature
78
What do you do to dehydrated culture media and dry ingredients?
Store at an even temperature in a cool dry place
79
What is the required storage temperature for plates of culture media, and additives like serum and blood in SOLID form?
2-8 degrees
80
What is the required storage temperature for solution form?
-2 degrees
81
It is essentual for the growth of strict anaerobes
Anaerobic atmosphere

Bact211 (10 decks)

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