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BACTE FINALS > VIBRIO > Flashcards

VIBRIO Flashcards

1
Q

Comma-shaped or curved bacillus

A

vibrio

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2
Q

vibrio species

A

● V. cholerae
● V.parahaemolyticus
● V. alginolyticus
● V. metschnikovii
● V. damsel and
● V. hollisae

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3
Q

Facultatively anaerobic and monotrichous organisms

A
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4
Q

Facultatively anaerobic and monotrichous organisms

A

vibrio

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5
Q

vibrio found on

A

brackish water, marine water, or salt water

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6
Q

vibrio isolated from

A

algae, plankton, fish and shellfish

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7
Q

vibrio is halophilic except for

A

V. cholera and V.mimicus

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8
Q

vibrio Mode of acquisition:

A

raw or undercooked seafood.

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9
Q

vibrio related infection

A

Cholera
wound
infection
septicaemia
necrotizing
fasciitis

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10
Q

Vibrio
Microscopy

A

Gram negative, short, curved and asporogenous rods.

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11
Q

Common Isolate of Vibrio

A

● Vibrio cholerae O1 and non O1
● Vibrio parahaemolyticus
● Vibrio vulnificus
● Vibrio alginolyticus

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12
Q

colonies are smooth, opaque and iridescent with greenish hue

A

Vibrio on CAP

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13
Q

colonies are NLF (except: V.vulnificus, w/c is lactose fermenter)

A

Vibrion on MAC

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14
Q

colonies are Alpha or Beta hemolytic

A

Vibrio on BAP

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15
Q

Susceptibility test use in vibrio

A

150 ug vibriostatic O/129 disk in MHA
They are susceptible

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16
Q

all specie ferment

A

glucose

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17
Q

all specie except are non lactose fermenter

A

V. vulnificus

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18
Q

all specie are oxidase ____ and reduce _____ to nitrite except

A

V. metschnikovii

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19
Q

Motility test:
Broth

A

polar sheated flagella;

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20
Q

Motility test:
Solid media

A

peritrichous, unsheated flagella.

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21
Q

Causative agent of cholera

A

VIBRIO CHOLERAE

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22
Q

Rapid darting or shooting-star motility

A

VIBRIO CHOLERAE

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23
Q

Virulence factor of Vibrio Cholerae

A

Choleragen (cholera toxin)

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24
Q

Potent enterotoxins of Vibrio cholerae

A

Cholera toxin,zot toxin and ace toxin.

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25
Q

colonies are smooth and medium to large sized greenish hue

A

vibrio cholerae on CAP

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26
Q

Common cause of cholera

A

V. cholerae O1

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27
Q

Antigenic structure of Vibrio cholerae

A

Somatic O and
Flagellar H

28
Q

● String test: Positive ● Other test:
● Oxidase: +
● Indole: +
● Lysine: +
● ODC: +

A

Vibrio cholerae

29
Q

V. cholerae subgroups

A

V. choleraeO1
V. cholerae O139
V. cholerae non-O1

30
Q

V. cholerae O1 serotypes

A

Ogawa (A, B),
Inaba (A, C) and
Hikojima (A, B, C)

31
Q

Epidemic V. cholerae O1 biogroups:

A

Classical and El Tor
● Classical O1 biogroups- susceptible to polymyxin B, Voges Proskauer Negative
● El Tor biogroups- resistant to polymyxin B, Voges Proskauer Positive

32
Q

● Acute diarrheal infection, spread through contaminated water source
● Ingestion of improperly preserved shellfish, milk and ice cream.

33
Q

Vibrio cholera

A

Hallmark: Rice watery stool

34
Q

V. cholerae O1

A

Common cause of epidemic

35
Q

Non-O1

A

Gastroenteritis

36
Q

String Test: Rgt

A

0.5% sodium desoxycholate

37
Q

Lysis of cells releases DNA, which can
then be pulled up into a viscous string

A

Positive reaction for String Test

38
Q

VIBRIO CHOLERAE ON TSI

A

A/A
(-) Gas
(-)H2S

39
Q

VIBRIO CHOLERAE ON LIA

40
Q

(+) citrate & indole

A

Vibrio cholerae

41
Q

(+) indole & cellobiose

A

V. vulnificus

42
Q

(+) indole

A

V. mimicus

43
Q

Second most common Vibrio species associated with gastroenteritis

A

Vibrio parahaemolyticus

44
Q

Etiologic agent of “summer diarrhea” in Japan

A

Vibrio parahaemolyticus

45
Q

MOA of vibrio parahaemolyticus

A

Eating contaminated seafood like oysters, scallops, crabs, lobsters, shrimps and sardines

46
Q

Leading cause of pandemic

A

V. parahaemolyticus serotypes O3:K6

47
Q

Virulence factor of Vibrio parahaemolyticus

A

Heat stable hemolysin

48
Q

Pathogenicity of V parahaemolyticus

A

Hemolysin lysis human RBC’s

49
Q

Selective medium for V parahaemolyticus

A

Wagatsuma agar (high mannitol medium

50
Q

 “Lactose positive” Vibrio species
 Second to V. cholerae as cause
of severe Vibrio-associated
infections

A

Vibrio vulnificus

51
Q

 Related infections: Septicemia
and wound infection
 MOA: Eating contaminated raw
oysters and fish

A

Vibrio vulnificus

52
Q

 Vibrio specie not commonly isolated and is considered as the least pathogenic to humans.
 Strict halophile that requires a medium with 1% to 10% Nacl

A

Vibrio alginolyticus

53
Q

Related infection: Eye, ear and wound infections (extraintestinal pathogen)

A

Vibrio alginolyticus

54
Q

Alkaline peptone water, thiosulfate- citrate-bile salts-sucrose agar (TBCS), MAC and BAP

A

Culture Media used in Vibrio

55
Q

Transport Medium in Vibrio

A

Cary-Blair medium

56
Q

Enrichment medium for V. cholerae

A

Alkaline
peptone water (pH 8.5)

57
Q

Growth of vibrios requires media that contain 0.5%
Nacl except

A

V. cholerae and V. mimicus

58
Q

tolerates upto 10% NaCl

A

V. alginolyticus

59
Q

grow as non-lactose fermenter
on MAC

A

Pathogenic vibrio

60
Q

TCBS inhibit

A

Gram-positive bacteria

61
Q

TCBS pH indicator

A

Thymol blue and parathymol blue

62
Q

Sucrose fermenters (Yellow colonies on TCBS)

A

V.cholerae, V. alginolyticus and V. metschnikovii

63
Q

Non-sucrose fermenter (Green colonies on TCBS)

A

V. mimicus, V. vulnificus, V. parahaemolyticus and V. damsel.

64
Q

 Used to separate vibrios (susceptible) from other oxidase-positive, glucose fermenter like aeromonads (resistant)
 Uses 150ug vibriostatic agent O/129 (2,4-diamino- 6,7-diisopropylpteridine.).

A

VIBRIOSTATIC TEST – O/129

65
Q

fail to agglutinate with O1 antisera

A

V. cholerae non-O1:

66
Q

serotyped by O and K antigen.

A

V. parahaemolyticus:

67
Q

150 ug vibriostatic O/129 disk orther name

A

2,4 diamino 6,7 diisopropylpteridine

BACTE FINALS (11 decks)

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