HELICOBACTER

Question 1

Gram neg, helical (S-shaped) rods,
resembles Campylobacter

Answer 1

Helicobacter

Question 2

Helicobacter Species

Answer 2

● Helicobacter pylori
● Helicobacter cinaedi
● Helicobacter rappini (formerly known as
Flexispira rappini)
● Helicobacter fenneliae

Question 3

● GIT of mammals and birds
● Motile by monopolar or multi bipolar
flagella
● Strong urease activity and are
microaerophiles

Answer 3

Helicobacter

Question 4

Helicobacter on CAP

Answer 4

colonies are gray and translucent

Question 5

Oxidase and catalase
____ on Helicobacter

Answer 5

positive

Question 6

Route

Answer 6

Oral-oral and Fecal-oral

Question 7

Gastric biopsy tissue, urine,
feces and dental plaque

Answer 7

Specimen

Question 8

Best specimen for the
culture of H. pylori

Answer 8

Gastric tissue

Question 9

utilized for ammonia
testing.

Answer 9

Urine specimens

Question 10

should be maintained at
4C and processed within 2 hours of
collection

Answer 10

Tissue specimen

Question 11

Major cause of type B gastritis, peptic
ulcer and gastric carcinoma

Answer 11

Helicobacter pylori

Question 12

Found in the mucous layer of the antrum
and fundus of the stomach but does not
penetrate gastric epithelium

Answer 12

Helicobacter pylori

Question 13

Binds to Lewis antigen and the
monosaccharide sialic acid.

Answer 13

Helicobacter pylori

Question 14

Primary habitat of H. pylori

Answer 14

Human gastric mucosa

Question 15

Primary habitat of H. pylori

Answer 15

Human gastric mucosa

Question 16

Biochemical test of H. pylori

Answer 16

Strong urease

Question 17

Diagnostic test: H. pylori

Answer 17

+) urea breath (sensitive
and specific).

Question 18

Isolated from the blood of the patient with
bacteremia and recovered from the blood of
homosexual males with and without HIV.

Answer 18

Helicobacter cinaedi and Helicobacter fenneliae

Question 19

enhances morphology

Answer 19

0.1% basic fuchsin counterstain

Question 20

Stain for biopsy

Answer 20

Warthin-Starry stain,
silver stain or Giemsa stain.

Question 21

Culture for H.pylori

Answer 21

CAP, MTM, Skirrow’s agar and brucella
agar with 5% sheep blood

Question 22

Transport media of H. pylori

Answer 22

Stuart medium, cysteine
brucella broth with 20% glycerol and isotonic
saline with 4% glucose

Question 23

should be
placed in a stuart medium

Answer 23

Gastric tissue biopsy specimen

Question 24

Helicobacter may require more than___ days of incubation in a capnophilic environment.

Answer 24

5 days

Question 25

sensitive method for detecting H. pylor

Answer 25

Nucleic Acid amplification (PCR)

Question 26

susceptible to metronidazole

Answer 26

H. pylori

Question 27

Susceptibility Testing: duration

Answer 27

3 days