BRUCELLA Flashcards
Small, coccobacilli arranged singly, in pairs or in
short chain, sandy-appearance, non-motile,
obligate aerobe
BRUCELLA SP (BANG’S BACILLUS)
o Brucella abortus
o Brucella suis
o Brucella canis
o Brucella melitensis
BRUCELLA SPECIES
- Human and animal pathogens
- Intracellular parasite
- Assacharolytic and non-encapsulated,
require an increased supply of CO2 for
growth. - Localized in tissue rich in erythritol
(placental tissue) and induces spontaneous
abortion among animals
BRUCELLA
Preferred specimen for brucella
Blood and Bone marrow
small convex,
translucent, yellowish and nonhemolytic
Brucella on BAP
Normal temperature in the morning and then
followed by high temperature in the afternoon
and evening
UNDULANT FEVER
o Ingestion of unpasteurized and
contaminated milk or cheese from
infected animals
o Inhalation of air around animal
carcasses (aerosol infection)
o Penetration of ocular or oral mucosa
o Direct inoculation into bloodstream
Primary route of undulant fever
BRUCELLA
* Specimen
Blood, bone marrow, skin lesions
and placental tissue (causes abortion)
biosafety level 3
agent in a class III cabinet due to their aerosol
mode transmission.
Brucella species, handled as
Carbolfuchsin should be substituted for
safranin O to improve the gram stain
Gram Stain for brucella
BAP, trypticase soy agar (TSA), and
Castañeda’s medium
culture used in brucella
requires niacin or nicotinic
acid for growth but can be inhibited by
adding thionine dye.
B. abortus:
Isolate can be recovered after ____ days but may require prolonged incubation → ____days
7 days
30 days
Serum agglutination test (SAT):
Brucella
Positive
with > 1:160 titer
Brucella canis in Serological test
not detected
