CAMPYLOBACTER Flashcards
Specimen for Campylobacter
feces, rectal swab & blood.
less-preferred specimen
Rectal swab
Gray, flat, glistening and irregular with
“tailing effect” along the streak line or
“runny spreading” growth
CULTURE OF CAMPYLOBACTER
Campy-BAP, Butzler agar,
Skirrow’s medium and charcoal cefoperazone
desoxycholate agar (CCDA)
Selective Media in Campylobacter
Transport medium
Cary-Blair Medium
both enriched and
have added antibiotics
Butzler and Skirrow’s media
contains antibiotics and is selective for
Campylobacter species.
CCDA
two-week incubation may be
needed
Blood culture
Campy can be detected effectively by a
CO2 monitoring system
Campy blood
agar plate , base?
Brucella agar
10% sheep red blood cells
Campy blood
agar plate, antimicrobial agaent?
Vancomycin
Trimethoprim
Polymyxin B
Amphotericin B
Cephalothin
Skirrow’s, base?
Oxoid blood
agar base.
Lysed,
defibrinated
horse RBC
Skirrow’s, antimicrobial agaent?
Vancomycin
Trimethoprim
Polymyxin B
Butzler, base?
Meat extract and peptone
Horse blood, defibrinated
Butzler, antimicrobial agaent?
Bacitracin
Novobiocin
Cycloheximide
Colistin
Cefazolin
CCDA base?
Nutrient agar
Charcoal
Sodium desoxycholate
CCDA antimicrobial agaent?
Cefoperazone
Amphotericin B
Associated with diarrheal disease and bacteremia in humans and in children with recurring
gastrointestinal illness (abdominal cramps), endocarditis, and peritonitis
Arcobacter butzleri
Common cause of duodenal ulcers and type B gastritis; possibly a risk factor in gastric carcinoma
Helicobacter pylori
Gastroenteritis, bacteremia in immunocompromised patients
Campylobacter coli
Most common cause of bacterial diarrhea worldwide
Campylobacter jejuni
Gram negative, small, S-shaped rods or curved
CAMPYLOBACTER
Campylobacter jejuni
o Campylobacter fetus subsp. Fetus
o Campylobacter coli
o Camplylobacterlari
o Campylobacter sputorum
o Campylobacter concisus
o Campylobacter curvus
o Campylobacter rectus
SPECIES OF CAMPYLOBACTER
Slow growing fastidious and asaccharolytic
organism
CAMPYLOBACTER JEJUNI
It has a darting motility and is unable to grow in
media with a high salt concentration
CAMPYLOBACTER JEJUNI
- Most common cause of bacterial gastroenteritis
- Can cause septic arthritis among AIDS patient
CAMPYLOBACTER JEJUNI
MOA OF CAMPYLOBACTER JEJUNI
eating contaminated chicken and turkey
(does not multiply in foods)
optimum temperature for growth for campy
42C
Infective dose
> 10,000 organisms.
Causes bacteremia and rarely associated with
gastrointestinal illness.
CAMPYLOBACTER FETUS SUBSP. FETUS
Counterstain
CAMPYLOBACTER
Carbolfuchsin
HAnging drop prep
CAMPYLOBACTER
darting motility
If safranin
(CAMPYLOBACTER)
2-3 minutes
Faintly staining, Gram-negative,
small, curved or S-shaped rods.
Microscopy of Campylobacter
coccobacillary
Old culture
long spirals
or are seagull wing-shaped.
Enteric campylobacters in microscopy
Also an animal pathogen (cattle and swine) that
causes sterility and abortion
CAMPYLOBACTER SP
Most recognized antecedent cause of
Guillain-Barr’e syndrome
CAMPYLOBACTER SP
Microaerophilic, except for C. rectus and C.
curvus, which are both strictly anaerobes
CAMPYLOBACTER SP
Motile with the presence of single polar flagellum
and secretes oxidase
CAMPYLOBACTER SP
: Ingestion of contaminated water, poultry
and dairy products; handling of pets like dogs,
cats and birds (occupational hazard); and sexual
transmission.
MOA for Campylobacter
Enteric campylobacters
C. jejuni, C. coli and
C. lari
