Veterinary Medicine - Infectious diseases Flashcards
What disease is caused by Erlichia Canis?
CME - Canine Monocytic Ehrlichiosis
Canine Monocytic Ehrlichiosis (CME) - Blood Smear - Possible findings
Activated monocytes
In 5% of cases (Extremely low sensitivity) - Morulas of Ehrlichia can be found in the cytoplasm monocytes at the feathered edge
Canine Monocytic Ehrlichiosis (CME) - Name 3 predisposed breeds and to what particular stage of the disease are they predisposed?
German shepherd, Husky, Malamute
Canine Monocytic Ehrlichiosis (CME) - Which lab finding can be found across all disease stages?
Thrombocytopenia
Canine Monocytic Ehrlichiosis (CME) - How long after infection are anti-platelets antibodies formed?
5 Days
From what platelet count you might start seeing spontaneous bleeding due to thrombocytopenia?
<30,000
Canine Monocytic Ehrlichiosis (CME) - Diagnosis - How can serology be useful in determining Ehrlichia Canis is the cause for the dogs current illness?
Seroconversion (4x) over a period of 2 weeks
Ehrlichiosis - Diagnosis - In general serology is more useful for Ruling Out Ehrlichiosis (T/F)
True
Canine Monocytic Ehrlichiosis (CME) - Treatment - At what stage of the disease Steroids might be useful?
Chronic stage
Canine Monocytic Ehrlichiosis (CME) - What is the vector?
Tick - Rhipicephalus sanguineous
Canine Monocytic Ehrlichiosis (CME) - Transmitted transovarially/transstadially?
Transstadial
Canine Monocytic Ehrlichiosis (CME) - What is the target cell for Ehrlicia Canis
Macrophages\Monocytes
Canine Monocytic Ehrlichiosis (CME) - How long does it take for an infected tick to transmit E.Canis to an animal after it started feeding
3 Hours
Canine Monocytic Ehrlichiosis (CME) - In what season do you see the most cases of E.Canis (Acute stage)
Summer months
Canine Monocytic Ehrlichiosis (CME) - Incubation period
1 - 3 Weeks
Canine Monocytic Ehrlichiosis (CME) - Chronic - What are the main 2 causes of death?
Sepsis
Anemia
Canine Monocytic Ehrlichiosis (CME) - Acute phase - Clinical signs
Lethargy
Anorexia
Fever
Lynphadenomeglay
Splenomegaly
Petechiae, ecchymoses
Melena/hematochezia, Pigmenturia, Epistaxis
Uveitis
Lameness
Canine Monocytic Ehrlichiosis (CME) - Acute phase - Common lab findings
Leukocytosis/Leukocytopenia
Monocytosis
Thrombocytopenia (Mild-moderate)
Blood smear - Activated monocytes +- Morulas
Canine Monocytic Ehrlichiosis (CME - Acute phase - Duration
1-4 Weeks
Canine Monocytic Ehrlichiosis (CME - Sub-clinical phase - Duration
Months-years
Canine Monocytic Ehrlichiosis (CME) - Sub-clinical phase - Common lab findings
General progression towards Pancytopenia: Leukopenia
Possible anemia (mild non-regenerative)
Thrombocytopenia
Canine Monocytic Ehrlichiosis (CME) - Sub-clinical phase - Clinical signs
None
Canine Monocytic Ehrlichiosis (CME) - Chronic phase - Clinical signs
Lethargy
Anorexia
Fever
Pale mucosal membranes
Lymphadenomeagly
Splenomegaly
Petechiae, ecchymoses, Epistaxis
GI Signs
CNS signs
Uveitis
Retinal separation
Lameness
Canine Monocytic Ehrlichiosis (CME) - In what stage of the disease do most owners bring their dog to the vet for the first time (Acute/Sub-clinical/Chronic)
Chronic stage
Canine Monocytic Ehrlichiosis (CME) - Chronic phase - Lab findings
Pancytopenia (Anemia, Leukopenia, Thrombocytopenia)
Hypoalbuminemia
Hyperglobulinemia (Polyclonal)
Mild elevation of liver enzymes
Canine Monocytic Ehrlichiosis (CME) - Chronic phase - Bone marrow cytological findings
Hypocellular bone marrow
Lipid deposition replacing bone marrow
Canine Monocytic Ehrlichiosis (CME) - Chronic phase - What are the Immune-mediated elements of the disease?
Immune complex formation
Auto-agglutinin formation (Comb’s Positive)
Anti-platelet antibodies
Anti-nuclear antibodies
Canine Monocytic Ehrlichiosis (CME) - Antibodies are the main defense against CME (True/False)
False
They do more harm than good
Canine Monocytic Ehrlichiosis (CME) - How long does it take for thrombocytopenia to resolve after treatment has began? (Acute stage)
10-14 Days
Canine Monocytic Ehrlichiosis (CME) - Thrombocytopenia - 4 Mechanisms in which E.Canis can cause thrombocytopenia
Increased consumption (e.g. Vasculitis)
Sequestration in the spleen
Destruction (IMT)
Decreased production in the bone marrow (Chronic stage)
Canine Monocytic Ehrlichiosis (CME) - Diagnosis - Blood smear - In what percentage of cases can morulas be seen? In what section of the smear? In what stage of the disease?
4%
Feathered edge
Acute
Canine Monocytic Ehrlichiosis (CME) - Can CME be transmissible through blood transfusion?
Yes
Canine Monocytic Ehrlichiosis (CME) - Diagnosis - Best diagnostic method
Blood PCR
Canine Monocytic Ehrlichiosis (CME) - Treatment - Treatment of choice (Acute stage), Treatment for chronic stages, Monitoring
Doxycycline - 3-4 Weeks.
Chronic stage:
Fluids, Anti-pyretics
Anemia - Blood transfusion, Darbepoetin.
Leukpoenia - G-CSF
Broad-spectrum antibiotics (2nd infections due to leukopenia)
Immunosuppression (e.g. GC, +/- 2nd drug such as Cyclosporine).
CBC (Monitor thrombocytopenia) + PCR
Canine Monocytic Ehrlichiosis (CME) - Treatment - Epistaxis
Acepromazine (+/- Benzodiazepines)
Adrenaline-soaked gauze
Tranexamic acid
Yunnan Baiyao
Canine Monocytic Ehrlichiosis (CME) - Negative prognostic indicators
Severe anemia
Severe thrombocytopenia
Severe leukopenia
Prolonged aPTT
Hypokalemia
Canine Monocytic Ehrlichiosis (CME) - Prevention
Anti-tick treatment
Anaplasma Platys - Vector
Rhipicephalus sanguineous
Anaplasma Platys - Target cell of the parasite
Platelets
Anaplasma Platys - Hallmark clincal-pathological finding
Cyclic thrombocytopenia (every 10-14 days)
Anaplasma Platys - Can be transmissible through blood transfusion (T/F)
True
Anaplasma Platys - Treatment
Doxycycline for 3-4 weeks
Anaplasma Platys - Causes a more severe disease when comes with CME co-infection (T/F)
True
Anaplasma Phagocytophilum - Target cell of the parasite
Neutrophils
Anaplasma Phagocytophilum - Acute / Chronic Disease Mostly?
Acute
Anaplasma Phagocytophilum - Lab findings
Anemia
Thrombocytopenia (90% of cases)
Lymphopenia
Hypoalbuminemia
Hyperglobulinemia
ALP Increase
Anaplasma Phagocytophilum - Diagnosis - Is blood smear a sensitive tool to detect the parasite?
Yes. Parasite can be detected in majority of cases
Anaplasma Phagocytophilum - Diagnosis
Blood smear, PCR
Anaplasma Phagocytophilum - Treatment, Prevention
Doxycycline
Tick prevention
Mycoplasma haemofelis - Modes of transmission
Bites
Fleas (unproven)
Blood transfusion
Intra-utero
Mycoplasma - Main type seen on blood smears
Mycoplasma Haemofelis
Mycoplasma haemofelis - Pathogenesis
Extravascular hemolysis => Strong regenerative anemia
Mycoplasma haemofelis - Blood smear findings
Anisocytosis
Polychromasia
Spherocytosis
Nucleated RBCs
Howell-jolly bodies.
RBCs infected with Mycoplasma can also be seen occasionally
Mycoplasma haemofelis - Classic signalment and concurrent pathologies to look for in infected cats
Male cats coming in contact with other cats
Co-infections causing immunosuppression (e.g. FIV, FeLV).
Abscess - A frequent finding occurring several weeks before acute disease with mycoplasma
Mycoplasma in cats - What types are usually associated with sub-clinical infection?
Candidatus M. hemominutum
Candidatus M. turicensis
Mycoplasma haemofelis - Clinical signs in acute episode
Fever
Lethargy
Anorexia
Pale mucus membranes
Splenomegaly
Lymphadenomegaly
Occasionally jaundice
Tachycardia
Tachypnea
Mycoplasma haemofelis - Diagnosis - Is blood smear useful for detecting the parasite? Also, in what percentage of clinical cats can Mycoplasma be detected in blood smears?
Mycoplasma (Haemofelis) can be detected in blood smears. However, bacteremia is cyclic and can disappear after 2 hours. Also, even if collected in the bacteremic phase - When collected with EDTA, after a while the parasite dissociate from the RBCs and die off - another source for false negative
50% of cases. If suspicion is high - keep taking blood samples every couple of hours
Mycoplasma haemofelis - Diagnosis (Gold standard)
PCR
Mycoplasma - Transmissible through blood transfusion?
Yes
Mycoplasma - Treatment
Doxycycline for 3-4 weeks
Fluoroquinolones also considered effective
GC if IMHA is suspected \ No-response to GC
Blood Transfusion if indicated
Anti-Pyretics, Fluids
Mycoplasma - Prognosis
Good. Clinical Improvement can be seen after 2-3 days from start of treatment. May remain a sub clinical carrier for years or for life / may experience repeat episodes.
Babesiosis - Modes of transmission
Tick bite
Blood Transfusion
Dog bite (B.Gibsoni)
Intrauterine (Some Spp.)
Babesiosis - Mechanisms of hemolysis (4)
RBC Rupture (Intravascular hemolysis)
Complement-mediated
Extravascular hemolysis
IMHA
Babesiosis - Breed predisposition - B.Vogeli
Greyhound
Babesiosis - Breed predisposition - B.Gibsoni
Pitbull
Babesiosis - Factors that determine severity of disease
Age (Young - more severe)
Babesia species ( B.Rossi > B.Canis > B.Vogeli)
Breeds (Greyhound, Pitbull)
Concurrent disease\Immunosuppression\Neoplasia
Splenectomy
Co-infections
Babesiosis - Clinical signs
Fever
Tachycardia
Tachypnea
Anorexia
Lethargy
Pale Mucus Membranes
Splenomegaly
Lymphadenomegaly
Pigmenturia
Icterus
Babesiosis - Common CBC findings
Regenerative anemia
Thrombocytopenia (mild-moderate)
Main differential for babesiosis
Primary \ Secondary IMHA
Babesiosis - B.Rossi - Name of specific pathology only to B.Rossi
Cerebral Babesiosis
Babesiosis - B.Vogeli - Mild/Moderate/Severe disease? Detectable in blood smear?
Mild-moderate
Parasitemia usually detectable
Babesiosis - B.Canis - Mild/Moderate/Severe disease? Detectable in blood smear?
Mild-severe
Parasitemia doesn’t usually correlate with severity