VbrAeroCmpyHlco Flashcards
Gram neg
Facultative Anaerobes
Curved/ Comma shaped RODS
Motile and Oxidase + except
Vibrio
V. metchnikovii - NM, Oxidase-
all species of Vibrio are Halophilic except
V. cholearae
V. mimicus
Vibrio spp are Glucose + Lactose -
except
V. vulnificus
Glucose-
Lactose+
DIAGNOSTIC for Vibrio
Specimens for Diagnosis
Transport media
Enrichment media
Selective-Differential media
Stool / Rectal Swabs
Carry - Blair
APW
TCBS CHOSucrose IndicatorsBromthymol Blue
Asiatic/ Epidemic Cholera
V. cholera
Virulence factor of V. cholerae
Motility
String test rgt
Choleragen/Cholera toxin
Shooting Star Motility
Sodium Desoxycholate
Green colonies on TCBS
‘’ m p v’’
V. mimicus, parahaemolyticus, vulnificus
Yellow colonies on TCBS
‘’ c a f f m’’
V. cholerae, alginolyticus, ,fluvialis, furnissi,metchnikovii
2nd most common Vibrio
Summer Diarrhea in JAPAN
Kanagawa phenomenon +(beta hemolysin due to tdh)
Agar use that has high salt mannitol
V. parahaemolyticus
Wagatsuma Agar
tdh- thermostable direct hemolysis
occupational hazard- continuous contact with Sea water
fishermen, sailor
V. alginolyticus
cause extraintestinal infections
SEPTICEMIA, MENINGITIS & WOUND INFECTION
Bull’s eye colonies in CIN
grow on MAC, EMB, SSA
Gram - Bacilli
BETA Hemolytic
Oxidase+
Aeromonas
S-shaped Bacilli
WINGS OF SEAGULLS
Darting motility
Hippurate+
Campylobacter
optimal temperature for growth of Campylobacter
CAPNOPHILIC
42-43 degC
causes febrile systemic syndrome
Gastroenteritis & Peridontal dse
Guillain Barre patients test positive for its antibodies
Campylobacter
MICROAEROPHILIC
Primary Isolation of Campylobacter
Modified SKIRROW’S MEDIA
CAMPY -BAP
CAMPY - CVA
Modified Charcoal Cefoperazone Deoxycholate agar
Formerly called Campylobacter coli
HUMAN GASTRIC MUCOSA
cause GASTRIC DUODENAL ULCER, GASTRIC CARCINOMA
Helicobacter pylori
Virulence factor of H. pylori
Urease production
makes the environment ALKALINE
TEST FOR IDENTIFICATION OF H. pylori
- Culture on urea broth agar: CONFIRMATORY
- a test that detect urease activity: SCREENING
- an immunochromatographic test(rapid):
- Tissue biopsy stain:
- invasive and costly:
Crushed tissue biopsy
Urea Breath test
COPRO STOOL ANTIGEN TEST
Warthin Starry
Endoscopy
primary disease caused by C. jejuni
Diarrhea
Campylobacter spp. causative agent of Bacteremia
C. fetus
rapidly progressive wound infection after exposure to contaminated water and septicemia after eating raw oysters
V. vulnificus
may show long filamentous form
Plesiomonas
Gelatin Hydrolysis +
Inositol fermenter
NaCI
String test +
Aeromonas and Vibrio
Plesiomonas
Vibrio
Vibrio
pag String test Positive SUSCEPTIBLE to PENICILLIN
Esculin Hydrolysis + Aeromonas spp
A. hydrophila, caviae