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Rj Flashcards

1
Q

MTB in SAMPLES

in purifying sputum -
in dried sputum -
droplets in air -
from culture and exposed to sunlight -
from sputum and exposed to sunlight -
in 5% phenol -
easily killed by -
in boiling -

A

weeks
6-8 months
8-10 days
2 hours
20-30 hours
24 hrs
moist heat (Autoclave)
10 minutes

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2
Q

AUtoclave control organisms

B. atrophaeus formerly B. subtilis var niger and B.globigii-
B. pumilus and B. subtilis
B. subtilis and B. atrophaeus

A

ETO STERILIZERS
IONIZING RADIATION
DRY HEAT OVEN

Dry SubAt
PumiLis ng Radiation glass

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3
Q

Medical waste autoclave temp

A

131degC
30-60 min
20 lbs

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4
Q

Visualized in Direct fluorescence antibody test

A

C. trachomatis elementary bodies infectious form of chlamydia

Non-Infectious: RETICULATE

Gonorrhe pili : 2% PTA
Typhi motility: Mannitol Motility medium
Agalactiae: Todd hewit broth

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5
Q
  1. Macrophage containing AFB, Foamy or lipid-laden macrophages
  2. hallmark of lepromatous leprosy
A
  1. Lepra cells
  2. Virchow cells
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5
Q

all about LEPRA CELLS

  1. oxygen req. of M. leprae
  2. Less severe form
  3. More contagious
  4. reservior
  5. common site
  6. Transmission
  7. Symptoms
  8. Diagnostic methods
  9. Co-vaccine
A
  1. Obligate Aerobe
  2. Tuberculoid
  3. Lepromatous
  4. Armadillo
  5. FIngers, Toes, Nose, Skin
  6. Direct contact w/ Nasal secretion, Skin contact sharing beddings
  7. Hypopigmentation, Reddish skin lesions w/ loss of sensation
  8. Acid fast staining, Culture footpads of mice
  9. BCG
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5
Q

a systemic pyogranulomatous infxn usually caused by inhalation of (spores)conidia of Blastomyces dermatitidis

A

Blastomycosis

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6
Q

Differentiation of Blastomyces vs. Histoplasma

A

AB TESTING AND DNA

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6
Q

description to take note of

  1. RUSSIAN DOLL
  2. Mother-Daughter cells
  3. An appendages that is longer than wide in serum
  4. A large, thick-walled survival spores in corn-meal agar with tween 80 and trypan blue
  5. several sausage-like appendages
  6. grape clusters along hyphae; at points of branching
A
  1. E. histolytica
  2. Blastomyces dermatitidis
  3. Germ tube
  4. Clamydospore
  5. Pseudohyphae
  6. Blastoconidia
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7
Q

Notable bacterial shapes/ arrangements/colonies

  1. Coffee bean or kidney shaped:
  2. Fried egg:
  3. Pearl like/ mercury droplet like:
  4. Chinese letter/picket fence:
  5. Cigar shaped:
  6. School of red fish:
  7. Comma shaped and fish in the stream appearance:
  8. Lancet shaped: dome-type (young): umbilicated (old):
  9. Safety pin:
  10. Necklace:
  11. Lollipop, drumstick, tennis racket:
  12. Boxcar, bamboo or thick squares:
  13. Diphtheroid-like/ palisades:
  14. Beaded rods (with warty, rough or cauliflower colonies):
A
  1. Neisseria
  2. M. hominis, S. mOniliformis, F. varium
  3. B. pertussis
  4. C diphtheriae
  5. M. leprae
  6. H. ducreyi
  7. V. cholerae
  8. S. pneumoniae
  9. Y. pestis
  10. S. moniliformis FRIED EGG
  11. C. tetani
  12. B. anthracis MEDUSA HEAD LION HEAD
  13. L. monocytogenes
  14. MTb
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7
Q

Notable bacterial shapes/ arrangements/colonies

  1. Poached egg colonies:
  2. Ground-glass colonies:
  3. Stalactite colonies:
  4. Daisy head:
  5. Mannitol golden yellow and pinhead:
  6. Fish eye colony:
  7. Pitted agar:
  8. AM-Waxy:
  9. Maple leaf:
  10. Coalescent and mucoid:
  11. Dew-like colonies with satellite phenomenon:
  12. Mulberry:
  13. Irregular:
  14. Irregular and feathery:
A
  1. C. diphtheriae
  2. B. subtilis and V. cholerae YELLOW GROUND GLASS: C. difficile
  3. Y. pestis
  4. C. Diphtheriae var gravis
  5. S. aureus
  6. Enterobacter
  7. Moraxella
  8. Proteus
  9. Salmonella.
  10. Klebsiella, S. pneumoniae
  11. H. influenza no satellite: H ducreyi
  12. Mycoplasma and PPLOs (pleuro-pneumonia-like organisms) NO CELL WALL
  13. Arizona
  14. P. aeruginosa
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8
Q

Special stains

  1. Gimenez and Machiavelli:
  2. Malachite green:
  3. Diene’s:
  4. Wayson’s: Polar bodies
  5. Albert’s and LAMB:
  6. Seller’s:
  7. India ink:
  8. Schaeffer fulton: ..
  9. Fite-Faraco:
  10. Leifson’s: ,
  11. Warthin starry, levaditi and Fontana:
  12. Dieterle’s:
A

1.Rickettsia
2. Ascaris eggs, C. diphtheriae, Endospores
3. Mycoplasma
4. Yersinia pestis
5. C. diphtheriae
6. negri bodies-rabies - ADELCHI NEGRI
7. Capsules
8. endospores
9. M. leprae
10. Flagella
11. Spirochetes
12. L. pneumophila

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8
Q

Pseudomonas Inclusion functions

  1. iron uptake
  2. siderophore for scavenging iron and ** signaling molecule** for the expression of virulence factors
  3. activity against bactericidal and antibiotics
  4. diminishes the oxidizing stress of the host microorganism tolerance against H2O2
A
  1. Pyocyanin
  2. Pyoverdin
  3. Pyorubin
  4. Pyomelanin
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9
Q

Bacterial Odors

  1. Fish-like:
  2. Garlic:
  3. Pungent:
  4. Musty:
  5. Bleach-like:
  6. Horse manure:
  7. Grape-like, Fruity and Corn Tortilla:
  8. Apple-like/sweet:
  9. Burnt gunpowder or ammoniacal:
  10. Freshly-cut apples:
  11. Dirty sneakers:
  12. Fecalent/putrid:
  13. Fruity:
  14. Wet-fur:
  15. Acrid:
  16. Butterscotch/ caramel:
  17. Mousy, Musty basement, mouse nest:
A
  1. V. cholerae
    2.C. diphtheriae. (DIPHT sa GARLIC)
  2. S. odorifera, P. multocida (PUNGENT ang ODOR sa mga MULTO)
  3. S. odorifera, Nocardia, Streptomyces (MUSTY nag ODOR ni STREP NO?)
  4. H. influenza, E. corrodens
  5. C. difficile (DIFFIcult to ride a HORSE)
  6. P. aeruginosa
  7. Achromobacter odorans
  8. Proteus
  9. A. faecalis,
  10. Staphylococcus, Citrobacter
  11. C. difficile, P. anaerobius
  12. Corynebacterium
  13. H. influenzae
  14. Pigmented bacteroides
  15. Viridans, Granulicatella, Milleri Strep
  16. H. influenzae MMM
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10
Q

a fungicide widely used in biomedical research to inhibit protein synthesis in EUKARYOTIC CELLS studied IN VITRO

1.SDA composition :DPD
2.. First usage
3.. Usual pH
4.. Purpose of PEPTONE
5.. Purpose of DEXTROSE
6.. SDA w/ Neutral pH

A

CYCLOHEXIMIDE

  1. DEXTOSE, PEPTONE AGAR, DISTILLED WATER
  2. DERMATOPHYTES
  3. 5.6 +/- 0.2 at 25degC
  4. Nitrogen and Vitamin source
  5. Carbon source
  6. Emmon’s
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11
Q

BACTEC BACSICS

  1. Volumes:
    Adults ; Children/Infants ; Infants/Neonates
  2. Bottle sets
    Adults ; Children
  3. Site Disinfectants:
  4. NOT recommended for Children < 2 months old
  5. inhibits the antibacterial effect of Serum and Phagocytes
  6. If the blood is immediately added to a sufficient vol. of 50mL of broth
  7. requirement for submitting catheter tips
  8. Prior to incubation, NEVER
  9. MOST popular continuous-monitoring blood culture systems are
A
  1. V: 20 mL ; 2-5 mL ; 1-2 mL
  2. BS: 2-3 ; 1-2
  3. 2% Tincture of Iodine, 10% Povidone iodine, 70% alcohol or 0.5% chlorhexidine in 70% alcohol chlorhexidine
  4. Chlorhexidine
  5. SPS
  6. NO AC NEEDED
  7. accompanying blood culture obtained by venipuncture
  8. REFRIGERATE
  9. BACTEC (Becton Dickinson and company, BacT/ALERT (bioMerieux) VersaTREK (thermo Scientific)

MANUAL: API > bioMerieux > CARBOHYDRATE UTILIZATION CHROMOGENIC SUBSTANCE
AUTOMATED: BD PHEONIX > BD DIAG. SYS. > CUCS/Fluorogenic susbstrate

12
Q

METHODS OF SUSCEPTIBILITY TESTING

  1. Dilution, Diffusion, Gradient, chromogenic, automated devices
  2. PCR, qPCR, cycle sequencing and Next gen Sequencing
  3. (maldi-tof) MATRIX ASSISTED LASER DESORTPTION IONIZATION - TIME OF FLIGHT
A
  1. PHENOTYPIC
  2. MOLECULAR
  3. MS-MASS SPECTROMETRY

MICROBIO (12 decks)

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