Biological Safety Levels Flashcards
BSL, Agent of Bioterrorism, Classification by RISK GROUPS
What BSL LEVEL
Those that can pose MINIMAL threat
no known potential of infecting healthy people
BSL 1
What BSL LEVEL
pose MODERATE threat
acquired through ingestion, percutaneous & mucous membrane exposure
associated with laboratory acquired infections
BSL 2
What BSL LEVEL
**HIGH RISK **
aerosol transmission
BSL 3
What BSL LEVEL
EXTREME RISK
causes life threatening diseases
BSL 4
what type of BSL
B. subtilis, M. gordonae, N gruberi
1
what type of BSL
Salmonella, Shigella, S. aureus,, HBV, HIV
2
what type of BSL
MTB
agent of Systemic Mycoses: H/ capsulatum, C. immitis
3
what type of BSL
*Filoviruses
* *Ebola virus
Crimean Congo Hemorrahagic fever virus
Arena viruses –LCM
4
BSL Precautions:
PPE + Sterile technique
BSL 1
BSL Precautions:
PPE, Sterile techniques, contact precautions vaccination use of Biohazard sign decontaminare all infectious waste
2
WHAT BSL PRECAUTIONS
Laboratory must have sustainable airflow in which air is drawn from clean area of the lab towards the contaminated area
3
WHAT BSL PRECAUTIONS
Class III BSC maximum containment Personnel and all materials must be decontaminated before leaving the facility
BSL 4
Agent of BIOTERRORISM what CATEGORY
easilytransmitted and can cause high mortality rate
Category A
Agent of BIOTERRORISM what CATEGORY
B. anthracis, C.botulinum, Y. pestis, F.tularesis
A
Agent of BIOTERRORISM what CATEGORY
Brucella, E. coli 0157:H7, Alpha viruses
B
Agent of BIOTERRORISM what CATEGORY
Hanta and Nipah Viruses
C
CLASSIFICATION OF INFECTIVE MICROORGANISMS by RISK GRP
A microorganism that is unlikely to cause human or animal disease
No or Low Individual and COmmunity Risk
Risk Group 1
CLASSIFICATION OF INFECTIVE MICROORGANISMS by RISK GRP
can cause human or animal disease but unlikely to be a serious hazard to lab workers
preventive measures are available
Moderate Individual risk - Low community risk
RG 2
CLASSIFICATION OF INFECTIVE MICROORGANISMS by RISK GRP
does not ordinarily spread from one ifected individual to another
High Individual risk- Low Community risk
but can cause serious human or animal dse
RG 3
CLASSIFICATION OF INFECTIVE MICROORGANISMS by RISK GRP
transmitted from one individual to anotherdirectly ot indirectly
effective treatment and preventive measures NOT USUALLY AVAILABLE
HIGH INDIVIDUAL RISK AND COMMUNITY RISK
RG 4
HEPA filters can remove objects larger than
0.3 um
>
Commonly used in BSC in the laboratory
CLASS 2
MOST Effective BSC
CLASS 3
LEAST Effective BSC
CLASS 1