Types of MEDIA Flashcards
Non-selective support the growth of wide range microorganism
Nutritive media
BAP, CAP
Nutritive media can also be
in that organism can be ditinguish basis of certain growth charac.
Differential media
considered both nutritive and differential medium
BAP
support ONE group of organism not another
by adding antimicrobials, dyes or alcohol to a particular medium
Selective medium
Supplemental or enrichment broth detect small numbers of organisms that may NOT grow in Solid primary media
2 most common
Back-up Broth
TSB, BHI Fastidious/Non-fastidious
THIO Aerobic/Anaerobic
proper media for growth of common CSF agents
CSF agents ‘‘S H E N B ‘’
BAP/ CAP
S pneumoniae, H influenzae, E coli, N meningitidis, B hemolytic Strep
Special type composed of liquid and solid form
the innoculum is added to the
when subcultures are to be made, the bottle is:
to allow the LIQUID to flow over the SOLID medium
Biphasic
LIQUID
TILTED
Castaneda bottles
ang agar naa sa side sa bottle
Number of bacteria per/mm broth needed for turbidity detected by the naked eye
10^6 - 1,000,000
THIO broth growth location
slightly below the surface where oxygen concentrations are lower than atmospheric concentrations
MICROAEROPHILIC
most common Solidifying agent
unique property of melting at high temp of
but re-solidify only after its temp falls below
Agarose
>95 degC
50 degC
temperature range for cooling solid media ofr distribution on petri dishes
55-60 deg C
resulting bacterial population is considered to be derived from a single bacterial cell and is known as
Pure colony
inhibitors for BBE Bacteroides Bile Esculin agar
Bile salts
Gentamycin
inhibitors for MAC inhibits GRAM POS
Crystal violet
Bile salts
