AIML Flashcards by Yormae Quezon

Mod. AF Stain

Decolorizer: Acid alcohol (H2SO4)

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Recover and identify CLostridium

Ethanol Shock test

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Mycobacterium spp. causes Pulmonary infection resembles MTB
recovered from HOT WATER TAPS and Contaminated Water System

M. Xenopi

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Detection of Lipase & Lecithinase Production

Egg Yolk agar/ Modified Mc Clung’s or neomycin agar

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Mehod of choice for the diagnosis of Lyme Disease

Serologic test IF or EIA

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U agar w/ Urea and Phenol red is for

Ureaplasma Urealyticum

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Medium of choice for the Selective recovery of Gram negative ANAEROBES

KANAMYCIN-VANCOMYCIN LAKE BLOOD

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Mycobacterium isolated from Subcutaneous skin (+) growth at 30 degC

*M. marinum
M. ulcerans
M/ haemophilum*

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on XLD

Colorless colonies
Redcolonies w/ Black centers
Yellowcolonies

*Shigella
Salmonella
EKE*

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Common Laboratory Acquired Infections

‘‘Hepa Sal TB Shig Bruce’’

Hepatitis
SAlmonellosis
TB
Shigelloides
Brucellosis

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it is done 40-60 seconds
most important practice to prevent spread of infection

HANDWASHING

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More susceptible to infection

Warm moist skin

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Variables that may change the MIC

Inoculum size
Incubation time
Growth rate of bacteria

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CSF and BLOOD should always be kept at

35-37 deg C

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Member of Enterobacteriaceae that most commonly produce ESBL

E. coli, K. pneumoniae

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the only Non-fermenter that produces abundant H2S on KIA or TSI

Shewanella putrefasciens

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M agar with Arginine and Phenol red is for

Mycoplasma

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MTB cultures are held in the Lab after identification and AST for

6 months

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Back-up Broth are also called

LIM BROTH Is for

Supplemental Broth

S. agalactiae

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DOH Memorandum No. 2023-0021

Sputum for TB Detection
Dx spx in 1 day

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Plan that identifies task that are hazardous to employees and promote safety to employees

This is responsibility of

EXPOSURE CONTROL PLAN

Micro Supervisor + Lab Director CMT

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Flexible Calcium Alginate Nasopharyngeal Swab is the device of choice for

C. diphtheriae

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CAP can provide X and V factor for

Haemophilus

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CCFA w/ Neutral Red will make colonies of C. diffcile

Yellow
because of fructose fermentation

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**VP test** detects which products of *glucose fermentation*

**Acetoin** or **Acetyl methyl carbinol**

**Amine product** of *Ornithine decarboxylation*

Putrescine

Organism that produce **YELLOW slant** on ***TSI*** but **RED slant** on ***KIA*** | '' S E C"

* **Serratia Edwardsiella Citobacter**

*Most commonly* implicated in **DIARRHEAL DISEASE**

# **IMViC** rections for the organisms * E. coli * S. tyhi * Y. enterocolitica * P. vulgaris

+ + - - - + - - V + - - + + - -

Difference between *PAE and S. maltophilia*

**OXIDASE, 42degC growth & Polar Tuft of Flagella** | PAE: Oxidase+, grow at 42, MONOtrichous

Produces **wrinkled colonies** but differentiated thru BIOCHEM TEST

*P. stutzeri B. pseudomallei*

aka. **acetyl trimethyl ammonium bromide agar**

**Cetrimide agar**- *PAE*

**Pyocyanin**(blue pigment) of PAE can be noted on

MHA

to differentiate **non-pathogenic** *Moraxella* growing on selected media for *Neisseria* from *Neisseria spp* use

10 units penicillin disk Moraxella are RESISTANT Neisseria are SUSCEPTIBLE

**Gram (+) SLENDER RODS** growing on **TOMATO JUICE AGAR**

*Lactobacillus*

**Manganous chloride Urea test** is rapid test for

*U. urealyticum* **(+) brown precipitates around the colonies**

**Resistant** of *N. gonorrhoeae* to **Penicillin** is due to

Beta lactamase

Collection of Urine from patients w/ **Indwelling catheter**

Aspirate aseptically from the catheter tubing

**SPS** is the AC fro Blood Culture bec.

It inactivates neutrophils & components of serum complement

**AST method** recommended to detect **Vancomycin Intermediate** *S. aureus*

**MIC Broth Dilution method**

Effect of placing antibiotic disk after organism inoculation and incubating within 15 minutes after disk placement

Decreases zone diameter | antibiotic disks should be placed within 15 minutes of inoculation and i

**Mistaken** for *N. gonorrhoeae* in **Gram neg** stained smears from **Uterine cervix exudate**

Moraxella osloensis

**Antimicrobial combination** uselful in confirming presence of **ESBL** in *E. coli*

Ceftazimide and Clavulanic acid

SARS virus was discovered in

China 2003

**Yeast enzyme** detected by birdseed agar

Phenol oxidase

Produces only **Blastoconidia**

*Cryptococcus*

produces **pseudohyphae and blastoconidia**

*Candida*

produces **Pseudohyphae, blastoconidia and arthroconidia**

*Trichosporon*

produces **Pseudohyphae and Arthroconidia**

*Geotrichum*

produces **Ascospores**

*Saccharomyces cerevisiae*

A rare cause of **Cat scratch disease**

Afipia felis

**test of choice for the confirmation** of *Clamydia trachomatis* infection in **URINE**

**PCR Molecular testing**

**most reliable** for the detection of *Mycoplasma pneumonia* in **serum** and for the **confirmation of diagnosis**

**EIA** and **DIrect Antigen Testing**

Features in which **MOLDS** are routinely categorized

* Macroscopic growth characteristics * Microscopic morphology

**Most sensitive test** for the initial diagnosis of *Cryptococcal disease*

**Cryptococcal Antigen**

Microscopic structure that are useful in the identification of **Dermatophytes**

**Macro and Microconidia**

Most useful in distinguishing **Yeast morphology**

**Corn meal agar with Tween 80**

**Encapsulated fungus** associated with **Eucalyptus tree**

*Cryptococcus gattii*

Specimen of choice for **RSV detection**

Nasopharyngeal aspirate

Most common **Anaerobic bacteria** isolated from infectious process of **Soft tissues and bacteremia**

*Bacteroides fragilis*

Most **sensitive** for the recovery of *Brucella* in **Chronic infection**

**Bone marrow**

**HACEK group** requires

**5-10% CO2 for growth**

acceptable AC for **Blood, BM, and Synovial fluid** that are to be cultured

* SPS * Heparin

Universally Susceptible to Penicillin

*S. pyogenes*

Methods of water bacteriologic exam

1. Physical 2. Chemical 3. Bacteriologic

# Methods of water bacteriologic exam: BACTERIOLOGIC * **Presumptive test** * **Confirmatory test** * **Completed test**

* Lauryl Sulfate Broth * EMB/ NA * Brilliant Green Bile Lactose Broth

DONNING | GMGG

**GOWN - MASK - GOGGLES/FACE SHIELD - GLOVES**

DOFFING

GLOVES - GOGGLES - GOWN - MASK *then do handwashing after*

a type of precaution applied to all **Human Blood & Body Fluids** that contain visible blood

**Universal precaution**

# water bacteriologic exams **Gold standard- multiple fermentation tube mtd**

Bacteriological (Presumptive-Confirmatory-Completed tests)

to treat patient's blood and Bloody fluid as **Potentially Infectious**

**STANDARD Precautions**