Urine cytology and CSF analysis Flashcards
What is better for detection of bacteria- wet mount or dry mount urine cytology?
dry mount
This sample was submitted for a urine cytology. What is the likely bacteria from? What is the organism called in this picture?
contamination from the GI tract
Cynicloyces guttulatus
Which sample site for CSF has higher protein- AO jt or LS jt?
LS
Where should CSF be collected if you suspect a lesion in the brain?
AO jt (always distal to the lesion)
What are contraindications for the collection of CSF fluid?
infection, increased ICP, kiari-like malformation, anesthetic concerns, coagulopathy
Normal protein concentration in CSF fluid in dogs/cat vs. horse?
What is the normal level of WBC and RBC in CSF?
WBC <5
RBC 0
Within how much time do you need to process CSF samples or preserve them?
60 minutes
What is the dominant cell type in CSF?
mononuclear cells (more lymphs)
What two preservatives can be used for CSF fluid?
hetastarch (1:1) and serum (1 drop/0.25 mL)
what results in a microprotein increase but normal cell counts in CSF fluid?
Damage to BBB
Obstruct CSF flow
cause localized tissue damage/necrosis
Increased protein concentration w/ CNS (neoplasia, infectious)
What are causes of increased protein conc., normal cell # but increased % of NT?
Do not involve meninges (IVDD, cervical stenotic myelopathy, spinal fractures, severe seizures, CNS necrosis)
blood contamination
prior steroid/abx use
increased cell counts in the CSF fluid is called ____?
pleocytosis
What is your top ddx for neutrophilic pleocytosis in the CSF?
What is another common ddx in dogs?
in horses?
bacterial meningitis
steroid-responsive meningitis arteritis
arborviruses (EEE)
Classify the CSF cytology.
Lymphocytic pleocytosis
What is the most common ddx for a lymphocytic pleocytosis?
What other differential should be considered?
viral meningitis
lymphoma
Classify the CSF cytology.
List possible ddx in dogs, cats, and LA?
Mixed cell pleocytosis
Dog: GME, chronic SRMA, infectious (fungal/protozoal)
Cat: chronic FIP
LA: Listeriosis ****
Classify the CSF fluid.
What is the most common DX for this in dogs? Which breed?
Eosinophilic pleocytosis
Eosinophilic steroid responsive meningitis
Golden retrievers
What is seen in this CSF fluid cytology?
What is the most likely cause of this?
myelin
aspiration of the spinal cord
Describe what is seen in each of the CSF samples.
distemper inclusion on left
lysosomal storage disorder on right
