Uric acid Flashcards
1
Q
Method
A
URICASE Quantitative Enzymatic Colorimetric Method (Stanbio)
2
Q
Principle
A
Uricase catalyzes the conversion of uric acid into allantoin and H2O2
The H2O2 is measured by its reaction with 3,5-dichloro-2-hydroxybenzenesulfonic
acid (DCHB) in the presence of peroxidase and 4-aminophenazone.
3
Q
Wavelength
A
520 nm
4
Q
End color
A
Red-violet (Quinoneimine complex)
5
Q
Conversion factor
A
0.0595 (59 → 60: senior, prone to gout)
6
Q
Conversion unit
A
F: 2.7–7.3 mg/dL || M: 4.0–8.5 mg/dL
7
Q
SI unit
A
F: 0.16–0.43 mmol/L || M: 0.24–0.51 mmol/L
8
Q
Product of which metabolism?
A
Purine metabolism
