HDL Cholesterol

Question 1

Method

Answer 1

POLYANION PRECIPITATION Method with ENZYMATIC Cholesterol Method
(Stanbio)

Question 2

Principle

Answer 2

Polyanion precipitation

Chylomicrons, Very Low-Density Lipoproteins (VLDL) and Low-Density
Lipoproteins (LDL) of serum are precipitated by magnesium chloride/dextran
sulfate.
After centrifugation, High Density Lipoproteins (HDL) is in the supernatant

Question 3

Wavelength

Answer 3

500 nm

Question 4

End color

Answer 4

Pink

Question 5

Reference values

Answer 5

HDL: 30- 75 mg/dL; LDL: 66- 178 mg/ dL