Alanine aminotransferase Flashcards
1
Q
Method
A
MODIFICATION OF IFCC AND BERGMEYER Method (Stanbio)
2
Q
Principle
A
Kinetic assay
ALT catalyzes the conversion of alanine and oxoglutarate into glutamate and
pyruvate.
Lacate dehydrogenase in the presence of NADH then converts pyruvate into
malate
3
Q
Wavelentgh
A
340 nm
4
Q
End products
A
Lactate and NAD+
5
Q
Conversion factor
A
3.376
6
Q
Reference values
A
3-35 U/L
