Alkaline phosphatase (ALP) Flashcards
1
Q
Method
A
BOWERS AND MCCOMB Method (STANBIO)
2
Q
Principle
A
Kinetic assay
ALP catalyzes the hydrolysis of p-nitrophenylphosphate at alkaline pH into p
nitrophenol and phosphate.
The p-nitrophenol produced forms a yellow chromogen when treated with a
dilute NaOH, the absorbance of which is directly p
3
Q
Wavelentgh
A
405nm
4
Q
End color
A
Yellow
5
Q
Conversion factor
A
2.764
6
Q
Reference values
A
34-114 U/L
