Bilirubin Flashcards
1
Q
Method
A
MODIFIED MALLOY-EVELYN Method (STANBIO)
2
Q
Principle
A
End point assay
Van den Bergh Reaction (Diazotization)
Bilirubin reacts with diazotized sulfanilic acid to form pink to purple Azobilirubin
3
Q
Wavelength
A
540 nm
4
Q
End color
A
Pink to purple azobilirubin
5
Q
Conversion factor
A
17.1
6
Q
Reference values
A
Direct Bilirubin – 0 to 0.5 mg/dL (0 to 8.55umol/L)
Total Bilirubin – 0 to 1.20 mg/dL (0 to 20.52umol/L)
