Lactate dehydrogenase Flashcards

1
Q

Method

A

BUHL AND JACKSON MODIFICATION OF WACKER Method (Stanbio)

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2
Q

Principle

A

Kinetic assay

LDH catalyzes the oxidation of lactate to pyruvate with the subsequent reduction
of NAD into NADH

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3
Q

Wavelength

A

340 nm

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4
Q

End products

A

Pyruvate and NADH

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5
Q

Conversion factor

A

3.376

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6
Q

Reference value

A

Male: 80-285 U/L
Female: 103-227 U/L

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