1
Q
Method
A
Modified Karmen Method
2
Q
Principle
A
Kinetic assay
AST catalyzes the conversion of aspartate and oxoglutarate into glutamate and
oxaloacetate.
Malate dehydrogenase in the presence of NADH then converts oxaloacetate
into malate
3
Q
Wavelength
A
340 nm
4
Q
End products
A
Malate and NAD+
5
Q
COnversion factor
A
3,376
6
Q
Reference values
A
8-33 U/L
CC 1 MSPE
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